Document Detail

Localization of angiogenic growth factors and their receptors in the human placental bed throughout normal human pregnancy.
MedLine Citation:
PMID:  19010534     Owner:  NLM     Status:  MEDLINE    
During early human pregnancy invasion of uterine spiral arteries by extravillous trophoblast cells contributes to their remodelling characterised by loss of musculo-elastic media and replacement by fibrinoid containing trophoblast. Despite its importance for successful pregnancy, the mechanisms underlying 'transformation' of spiral arteries are not well understood. The aim of this study was to localize expression of members of the angiopoietin (Ang) family (Ang-1, Ang-2 and their receptor Tie-2) and the vascular endothelial growth factor (VEGF) family (VEGF-A, VEGF-C, VEGF-D and their receptors VEGF-R1, VEGF-R2 and VEGF-R3) in the placental bed throughout normal human pregnancy. Placental bed biopsies were obtained from women undergoing elective termination of pregnancy at 8-10, 12-14 and 16-20 weeks' gestation and elective caesarean section at term (n=6 each group). Paraffin-embedded sections were immunostained for Ang-1, Ang-2, Tie-2, VEGF-A, VEGF-C, VEGF-D, VEGF-R1, VEGF-R2 and VEGF-R3 using an avidin biotin peroxidase technique. Reactivity of endovascular, interstitial, intramural and multinucleate extravillous trophoblast populations in the placental bed was analysed semi-quantitatively. There was an increase in the level of immunostaining of intramural EVT for Tie-2 and VEGF-C with increasing gestational age. In addition, there was a reduction in Ang-1 and Ang-2 expression by multinucleate interstitial EVT and of VEGF-R1 and VEGF-R2 by endovascular EVT with increasing gestational age. At the earlier gestational ages studied, immunostaining for Ang-1, Ang-2, Tie-2, VEGF-C, VEGF-R1 and VEGF-R2 on intramural EVT was reduced compared to both mononuclear interstitial and endovascular EVT. These findings suggest that the Ang and VEGF families may play a role in the process of spiral artery remodelling in normal pregnancy.
B Schiessl; B A Innes; J N Bulmer; H A Otun; T J Chadwick; S C Robson; G E Lash
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Publication Detail:
Type:  Journal Article     Date:  2008-11-17
Journal Detail:
Title:  Placenta     Volume:  30     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-23     Completed Date:  2009-03-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  79-87     Citation Subset:  IM    
School of Surgical and Reproductive Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
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MeSH Terms
Angiopoietins / metabolism*
Arteries / metabolism
Cesarean Section
Gestational Age
Immunoenzyme Techniques
Neovascularization, Physiologic / physiology*
Placenta / anatomy & histology,  blood supply*
Placental Circulation / physiology*
Receptor, TIE-2 / metabolism*
Trophoblasts / cytology,  metabolism
Vascular Endothelial Growth Factor A / metabolism*
Young Adult
Reg. No./Substance:
0/Angiopoietins; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; EC, TIE-2

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