Document Detail


Localization of ampicillin-sensitive sites in Escherichia coli by electron microscopy.
MedLine Citation:
PMID:  783143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growth of Escherichia coli B/r ATCC 12407 (doubling time, 65 to 70 min) in the presence of 500 mug of ampicillin per ml for 15 to 20 min induces a sphere alongside the cell. The position was determined with respect to the length axis of the cell by electron microscopy. Although spheres may be found anywhere, some prominent sites do occur. In the shortest cells, which have a length of about 1.5 mum, they are found at the presumed new cell pole. In slightly older cells (length, about 1.8 mum), the position of the sphere is not well defined. Later on spheres occur predominantly at the cell center. In dividing cells (average length, 2.5 mum) a sphere may also occur at about one-quarter of the cell length. The position of the spheres bears resemblance to sites where a pulse of 3H-labeled diaminopimelic acid is incorporated into the peptidoglycan, as has been found by others.
Authors:
P Staugaard; F M van den Berg; C L Woldringh; N Nanninga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  127     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-11-01     Completed Date:  1976-11-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1376-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Ampicillin / pharmacology*
Cell Division
Cell Wall / drug effects
Escherichia coli / drug effects*,  growth & development,  ultrastructure
Microscopy, Electron
Chemical
Reg. No./Substance:
69-53-4/Ampicillin
Comments/Corrections

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