Document Detail

Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression.
MedLine Citation:
PMID:  17686885     Owner:  NLM     Status:  MEDLINE    
Members of the Rh glycoprotein family have been shown to be involved in ammonia transport in a variety of species. Here we show that zebrafish Rhcg1, a member of the Rh glycoprotein family, is highly expressed in the yolk sac, gill, and renal tubules. Molecular cloning and characterization indicate that zebrafish Rhcg1 shares 82% sequence identity with the pufferfish ortholog fRhcg1. RT-PCR, combined with in situ hybridization, revealed that Rhcg1 is first expressed in vacuolar-type H(+)-ATPase/mitochondrion-rich cells (vH-MRC) on the yolk sac of larvae at 3 days postfertilization (dpf) and later in vH-MRC-like cells in the gill at 4-5 dpf. Ammonia excretion from zebrafish larvae increased in parallel with the expression of Rhcg1. At larval stages, Rhcg1 mRNA was detected only on the yolk sac and gill; however, the kidney, as well as the gill, becomes a major site of Rhcg1 expression in adults. Using a zebrafish Tol2 transgenic line whose vH-MRC are labeled with green fluorescent protein (GFP) and an antibody against zebrafish Rhcg1, we demonstrate that Rhcg1 is located in the apical regions of 1) vH-MRC on the yolk sac and vH-MRC-like cells (cell population with the expression of Rhcg1 and GFP) in the gill and 2) cells in the renal distal tubule and intercalated cell-like cells in the collecting duct of the kidney. Remarkably, expression of Rhcg1 mRNA at the larval stage was changed by environmental ionic strength. These results suggest that roles of zebrafish Rhcg1 are not solely ammonia secretion to eliminate nitrogen from the gill.
Tsutomu Nakada; Kazuyuki Hoshijima; Masahiro Esaki; Saori Nagayoshi; Koichi Kawakami; Shigehisa Hirose
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-08-08
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  293     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-03     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1743-53     Citation Subset:  IM    
Dept. of Biological Sciences, Tokyo Institute of Technology, 4259-B19 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa-ken, 226-8501 Japan.
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MeSH Terms
Amino Acid Sequence
Animals, Genetically Modified
Base Sequence
Cation Transport Proteins / chemistry,  genetics*,  metabolism*
Gene Expression Regulation
Gills / cytology*
Green Fluorescent Proteins
Kidney / cytology*
Mitochondria / metabolism*
Osmolar Concentration
Protein Transport
RNA, Messenger / genetics,  metabolism
Water / chemistry
Yolk Sac / cytology*
Zebrafish Proteins / chemistry,  genetics*,  metabolism*
Grant Support
Reg. No./Substance:
0/Cation Transport Proteins; 0/RNA, Messenger; 0/Rhcg1 protein, zebrafish; 0/Zebrafish Proteins; 147336-22-9/Green Fluorescent Proteins; 7732-18-5/Water

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