Document Detail

Localization and accumulation of fibronectin in rabbit fetal lung tissue.
MedLine Citation:
PMID:  3301486     Owner:  NLM     Status:  MEDLINE    
An interaction between mesenchyme and epithelium is required for the normal differentiation of fetal lung tissue. This morphogenic interaction may be mediated, in part, by changes in the composition and/or structure of the extracellular matrix. Therefore, we characterized the localization and accumulation of fibronectin, an extracellular-matrix component, during several stages of lung development in the rabbit fetus in vivo as well as in day-21 rabbit fetal lung explants maintained in vitro. Fibronectin was detected immunocytochemically in the basement-membrane zone beneath the epithelial ducts in lung tissue obtained from rabbit fetuses at 19 and 21 days of gestation. In fetal lung tissue obtained at these early stages of lung development, mesenchymal cells were stained only at their periphery. Immunostaining for connective-tissue fibronectin increased greatly between days 24 and 31 of gestation. A similar increase in the intensity of immunostaining for connective-tissue fibronectin was observed in rabbit fetal lung explants that had been maintained in vitro for 7 days. The concentration of fibronectin in fetal lung tissue obtained at different days of gestation was determined using a specific enzyme-linked immunoadsorbent assay (ELISA) and was found to increase from 1.7 ng/micrograms protein in fetal lung tissue obtained at day 19 of gestation to 7.3 ng/micrograms protein in fetal lung tissue obtained at day 24 of gestation. The levels of fetal lung fibronectin then remained relatively constant through to day 31 of gestation. A similar increase in fibronectin concentration was observed in day-21 fetal lung explants maintained in vitro for 7 days.(ABSTRACT TRUNCATED AT 250 WORDS)
J M Snyder; J A O'Brien; H F Rodgers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  34     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1987  
Date Detail:
Created Date:  1987-09-03     Completed Date:  1987-09-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  32-9     Citation Subset:  IM    
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MeSH Terms
Enzyme-Linked Immunosorbent Assay
Fibronectins / metabolism*
Fluorescent Antibody Technique
Gestational Age
Lung / cytology,  embryology*,  metabolism
Organ Culture Techniques
Grant Support
Reg. No./Substance:

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