Document Detail

Localization of Reg receptor in rat fundic mucosa.
MedLine Citation:
PMID:  11919548     Owner:  NLM     Status:  MEDLINE    
Reg protein has a trophic effect on gastric mucosal cells and pancreatic islets. Recently, the Reg receptor (Reg-R) has been cloned, and Reg-Reg-R interaction has been reported in the pancreas. The aim of this study was to investigate the localization of Reg-R in rat fundic mucosa. Gene expression of Reg-R was investigated with Northern blot analysis, laser capture microdissection coupled with reverse transcription-polymerase chain reaction, and in situ hybridization in the fundic mucosa, and the types of cells expressing this gene were determined. Reg-R mRNA expression was detected mainly in chief cells and parietal cells of the deep layers and faintly in surface epithelial cells and mucous neck cells of the proliferating zone. Our results suggest that regenerating protein may act not only as a regulator of gastric epithelial cell proliferation but also as a modifier of other multiple physiologic functions.
Hideaki Kazumori; Shunji Ishihara; Ryo Fukuda; Yoshikazu Kinoshita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  139     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-28     Completed Date:  2002-04-18     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  AIM; IM    
Second Department of Internal Medicine, Shimane Medical UniversityIzumo, Japan.
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MeSH Terms
Blotting, Northern
Calcium-Binding Proteins / physiology*
Cloning, Molecular
Gastric Fundus / chemistry*
Gastric Mucosa / chemistry*
Gene Expression
In Situ Hybridization
Nerve Tissue Proteins*
RNA, Messenger / analysis,  genetics
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Lithostathine; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Reg1 protein, rat

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