Document Detail

Localization of M2 muscarinic receptor protein in parvalbumin and calretinin containing cells of the adult rat entorhinal cortex using two complementary methods.
MedLine Citation:
PMID:  15708496     Owner:  NLM     Status:  MEDLINE    
We investigated parvalbumin (PV) and calretinin (CR) containing interneurons in the rat entorhinal cortex. RNA amplification following single cell dissection of immunohistochemically labeled cells from layers II to VI revealed that PV cells, in contrast to CR cells, express the m2 muscarinic receptor (M2AchR) protein. Double immunostaining to confirm the results of RNA amplification indicated that the majority of PV cells contain M2AchR protein, whereas only a small proportion of CR cells do. In contrast, a large number of layer I CR cells, which are mostly Cajal-Retzius cells, were positive for M2AchR. RNA amplification following dissection of these cells also revealed that they contain the M2AchR protein. These findings emphasize that there are significant differences in the expression of different proteins, even among similar neuronal types in the same brain region. This highlights the importance of accurately collecting single cells, and knowledge of anatomical details in molecular biological studies.
J D Chaudhuri; M Hiltunen; M Nykänen; S Ylä-Herttuala; H Soininen; R Miettinen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  131     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2005  
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  557-66     Citation Subset:  IM    
Department of Neuroscience and Neurology, University of Kuopio, PO Box 1627, Harjulantie 1, FIN-70211 Kuopio, Finland.
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MeSH Terms
Calcium-Binding Protein, Vitamin D-Dependent / analysis*
Entorhinal Cortex / chemistry*,  cytology*
Parvalbumins / analysis*
Rats, Wistar
Receptor, Muscarinic M2 / analysis*
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Parvalbumins; 0/Receptor, Muscarinic M2; 0/calretinin

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