Document Detail


Localization of label-retaining cells in murine vocal fold epithelium.
MedLine Citation:
PMID:  21330647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Epithelial homeostasis is critical for vocal fold health, yet little is known about the cells that support epithelial self-renewal. As a known characteristic of stem cells is that they are slow-cycling in vivo, the purpose of this prospective controlled study was to identify and quantify slow-cycling cells or putative stem cells in murine vocal fold epithelium.
METHOD: Twelve mice were administered daily intraperitoneal injections of a nucleotide dye, bromodeoxyuridine (BrdU), over 7 consecutive days. Under this pulse-chase paradigm, slow-cycling cells retain the dye (label-retaining cells; LRCs) while more rapidly cycling cells lose dye to dilution during multiple cell divisions. The percentage of label-retaining cells (%LRCs) was calculated following a chase period of 2, 4, and 8 weeks postinjections.
RESULTS: The %LRCs decreased significantly from 9.4% at 2 weeks to 3.1% at 8 weeks following injections (p < .05). No statistically significant differences in the quantity of BrdU-positive cells were measured between the anterior, mid-membranous, or cartilaginous regions of the vocal fold (p > .05).
CONCLUSION: These findings are consistent with the presence and first report of a small population of putative stem cells along the length of murine vocal fold epithelium.
Authors:
Ciara Leydon; Rebecca S Bartlett; Drew A Roenneburg; Susan L Thibeault
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-02-17
Journal Detail:
Title:  Journal of speech, language, and hearing research : JSLHR     Volume:  54     ISSN:  1558-9102     ISO Abbreviation:  J. Speech Lang. Hear. Res.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-02     Completed Date:  2011-12-28     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  9705610     Medline TA:  J Speech Lang Hear Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1060-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Cell Tracking
Epithelial Cells / cytology*
Immunohistochemistry
Larynx / cytology*
Male
Mice
Mice, Inbred C57BL
Stem Cell Niche / physiology*
Vocal Cords / cytology*
Grant Support
ID/Acronym/Agency:
R01 DC004336/DC/NIDCD NIH HHS; R01 DC004336/DC/NIDCD NIH HHS; R01 DC004336-13/DC/NIDCD NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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