Document Detail


Localization of Islet-1-positive cells in the healthy and infarcted adult murine heart.
MedLine Citation:
PMID:  22427341     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: The transcription factor Islet-1 is a marker of cardiovascular progenitors during embryogenesis. The isolation of Islet-1-positive (Islet-1(+)) cells from early postnatal hearts suggested that Islet-1 also marks cardiac progenitors in adult life.
OBJECTIVE: We investigated the distribution and identity of Islet-1(+) cells in adult murine heart and evaluated whether their number or distribution change with age or after myocardial infarction.
METHODS AND RESULTS: Distribution of Islet-1(+) cells in adult heart was investigated using gene targeted mice with nuclear β-galactosidase inserted into the Islet-1 locus. nLacZ-positive cells were only present in 3 regions of the adult heart: clusters in the interatrial septum and around the pulmonary veins, scattered within the wall of the great vessels, and a strictly delimited cluster between the right atrium and superior vena cava. Islet-1(+) cells in the first type of clusters coexpressed markers for parasympathetic neurons. Positive cells in the great arteries coexpressed smooth muscle actin and myosin heavy chain, indicating a smooth muscle cell identity. Very few Islet-1(+) cells within the outflow tract expressed the cardiomyocyte marker α-actinin. Islet-1(+) cells in the right atrium coexpressed the sinoatrial node pacemaker cell marker HCN4. Cell number and localization remained unchanged between 1 to 18 months of age. Consistently Islet-1 mRNA was detected in human sinoatrial node. Islet-1(+) cells could not be detected in the infarct zone 2 to 28 days after myocardial infarction, aside from 10 questionable cells in 1/13 hearts.
CONCLUSIONS: Our results identify Islet-1 as a novel marker of the adult sinoatrial node and do not provide evidence for Islet-1(+) cells to serve as cardiac progenitors.
Authors:
Florian Weinberger; Dennis Mehrkens; Felix W Friedrich; Mandy Stubbendorff; Xiaoqin Hua; Jana Christina Müller; Sonja Schrepfer; Sylvia M Evans; Lucie Carrier; Thomas Eschenhagen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-15
Journal Detail:
Title:  Circulation research     Volume:  110     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-14     Completed Date:  2012-07-10     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1303-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Aorta / cytology,  metabolism
Biological Markers / metabolism
Chromogenic Compounds / diagnostic use
Galactosides / diagnostic use
Indoles / diagnostic use
LIM-Homeodomain Proteins / genetics*,  metabolism*
Lac Operon
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myocardial Infarction / metabolism*,  pathology
Myocardium / cytology,  metabolism*
Pulmonary Artery / cytology,  metabolism
RNA, Messenger / metabolism
Sinoatrial Node / cytology,  metabolism*
Transcription Factors / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Chromogenic Compounds; 0/Galactosides; 0/Indoles; 0/LIM-Homeodomain Proteins; 0/RNA, Messenger; 0/Transcription Factors; 0/insulin gene enhancer binding protein Isl-1; V595OG374W/5-bromo-4-chloro-3-indolyl beta-galactoside
Comments/Corrections
Comment In:
Circ Res. 2012 May 11;110(10):1267-9   [PMID:  22581917 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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