Document Detail


Localization of DNA in Coxiella burnetii by post-embedding immunoelectron microscopy.
MedLine Citation:
PMID:  2378445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The deoxyribonucleic acid of Coxiella burnetii was detected with monoclonal antibodies against single-stranded and double-stranded DNA by post-embedding immunoelectron microscopy. The antibody labeled the nucleoid of the spore, the small cell variant (SCV), and the large cell variant (LCV). The DNA was segregated completely in both the spore and the mother cell. At the terminal stage of development of the spore, the loss of nuclear morphology in the mother cell was related to the reduction in the labeling of the nucleoid. The mother cell was therefore killed for the development and release of the spore.
Authors:
T F McCaul; J C Williams
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  590     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1990  
Date Detail:
Created Date:  1990-08-31     Completed Date:  1990-08-31     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  136-47     Citation Subset:  IM    
Affiliation:
United States Army Medical Research Institute of Infectious Diseases, Department of Intracellular Pathogens, Fort Detrick, Frederick, Maryland 21701-5011.
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MeSH Terms
Descriptor/Qualifier:
Coxiella / genetics*
DNA, Bacterial / analysis*,  immunology
Immunohistochemistry
Microscopy, Electron
Spores, Bacterial / analysis
Chemical
Reg. No./Substance:
0/DNA, Bacterial

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