| Localization of Ca++-containing antimonate precipitates during mitosis. | |
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MedLine Citation:
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PMID: 7400217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular bound Ca++ has been localized throughout mitosis and cytokinesis in two plant species by means of in situ precipitation with potassium antimonate and electron microscope visualization. Identification of Ca++ as the major cation precipitated was made by comparing solubility properties in water, EDTA, and EGTA of the intracellular deposits with respect to those of K+-, Mg++-, and Ca++-antimonate standards. In spermatogenous cells of the water fern, Marsilea vestita, and stomatal complex cells of barley, Hordeum vulgare, antimonate deposits have been found associated with the endoplasmic reticulum (ER), vacuoles, euchromatin/nucleoplasm, and mitochondria. The last contain a much higher density of precipitates in Marsilea than in Hordeum. Dictyosomes and the nuclear envelope of Marsilea also contain antimonate deposits, as do the plasmalemma, cell wall, and phragmoplast vesicles of Hordeum. Microtubule-organizing centers such as kinetochores and the blepharoplast of Marsilea do not stain. In spite of differences in associated antimonate between certain organelles of the two species, the presence of antimonate aong the ER throughout the cell cycle is common to both. Of particular interest are those precipitates seen along the tubules and cisternae of the extensive smooth ER that surrounds and invades the mitotic spindle in both species. The ability to bind divalent cations makes the mitotic apparatus (MA)-associated ER a likely candidate for regulation of free Ca++ levels in the immediate vicinity of structural components and processes that are Ca++-sensitive and proposed to be Ca++-regulated. |
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Authors:
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S M Wick; P K Hepler |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 86 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1980 Aug |
Date Detail:
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Created Date: 1980-10-27 Completed Date: 1980-10-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 500-13 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaphase Calcium / metabolism* Cell Division Cytoplasm / metabolism Endoplasmic Reticulum / metabolism Intracellular Membranes / metabolism* Microscopy, Electron / methods Microtubules / metabolism Mitosis* Organoids / metabolism Plants Telophase |
| Chemical | |
Reg. No./Substance:
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7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 8 Year: 1980 Volume: 86 Issue: 2 First Page: 500 Last Page: 513 ID: 2111497 Publisher Id: 80249662 PubMed Id: 7400217 |
| Localization of Ca++-containing antimonate precipitates during mitosis | |
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