Document Detail


Localisation of eIF4A-III in the nucleolus and splicing speckles is an indicator of plant stress.
MedLine Citation:
PMID:  19855185     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The mechanisms of long-term adaptation to low oxygen environment are quite well studied, but little is known about the sensing of oxygen shortage, the signal transduction and the short-term effects of hypoxia in plant cells. We have found that an RNA helicase eIF4A-III, a putative component of the Exon Junction Complex, rapidly changes its pattern of localisation in the plant nucleus under hypoxic conditions. In normal cell growth conditions GFP-eIF4A-III was mainly nucleoplasmic, but in hypoxia stress conditions it moved to the nucleolus and splicing speckles. This transition occurred within 15-20 min in Arabidopsis culture cells and seedling root cells, but took more than 2 h in tobacco BY-2 culture cells. Inhibition of respiration, transcription or phosphorylation in cells and ethanol treatment had similar effects to hypoxia. The most likely consequence is that a certain mRNA population will remain bound to the eIF4A-III and other mRNA processing proteins, rather than being transported from the nucleus to the cytoplasm, and thus its translation will be suspended.
Authors:
Olga A Koroleva; John W S Brown; Peter J Shaw
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2009-12-25
Journal Detail:
Title:  Plant signaling & behavior     Volume:  4     ISSN:  1559-2324     ISO Abbreviation:  Plant Signal Behav     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-10-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101291431     Medline TA:  Plant Signal Behav     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Cell Biology, John Innes Centre, Colney, Norwich, UK.
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