| Localisation of dopamine D2-like receptors in pulmonary artery of the human and rabbit but not of the rat. | |
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MedLine Citation:
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PMID: 7813543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was designed to investigate the presence of dopamine D2-like receptor sites in the main trunk of the human, rabbit and rat pulmonary artery using combined radioligand binding and light microscope autoradiography techniques. [3H]Spiroperidol was used as a ligand. The presence and the localisation of the sympathetic neuroeffector plexus were also studied using catecholamine histofluorescence techniques. Radioligand binding experiments demonstrated the labelling of a population of dopamine D2-like receptors in sections of human and rabbit pulmonary arteries by [3H]spiroperidol. No specific binding occurred in sections of the rat pulmonary artery. Light microscope autoradiography showed the development of specific silver grains within the tunica adventitia, including the adventitia-media border, of the human and rabbit pulmonary arteries. No specific silver grains were found in sections of the rat pulmonary artery. Studies on the pharmacological characterisation of [3H]spiroperidol binding sites in the human and rabbit pulmonary arteries showed that they are sensitive primarily to domperidone, haloperidol, (-)-sulpiride or bromocriptine, and to a lesser extent to n-propylnorapomorphine, quinpirole or clozapine displacement. This suggests that [3H]spiroperidol binding sites in the pulmonary artery probably belong to the dopamine D2 receptor subtype. Catecholamine histofluorescence techniques revealed a rich plexus of fluorescent adventitial and adventitial-medial nerve fibres in the human and to a lesser extent in the rabbit pulmonary artery. Comparison of the localisation of dopamine D2-like receptor sites and of the sympathetic neuroeffector plexus in the pulmonary artery, suggests a possible prejunctional localisation of these sites. |
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Authors:
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Y Kobayashi; D Cavallotti; A Ricci; F Amenta |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of pharmacology Volume: 261 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-02-09 Completed Date: 1995-02-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 229-36 Citation Subset: IM |
Affiliation:
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Sezione di Anatomia Umana, Università di Camerino, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Animals Autoradiography Catecholamines / metabolism Fluorescent Antibody Technique Histocytochemistry Humans Male Neuroeffector Junction / drug effects Pulmonary Artery / anatomy & histology, innervation, metabolism* Rabbits Radioligand Assay Rats Rats, Wistar Receptors, Dopamine D2 / metabolism* Species Specificity Spiperone / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Catecholamines; 0/Receptors, Dopamine D2; 749-02-0/Spiperone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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