Document Detail


Localisation of cyclooxygenase 1 and cyclooxygenase 2 in Helicobacter pylori related gastritis and gastric ulcer tissues in humans.
MedLine Citation:
PMID:  10807888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Prostaglandin endoperoxide synthase/cyclooxygenase (COX) is the key enzyme in gastric mucosal protection and repair but its cellular localisation in the human stomach is still unclear. AIMS: To investigate immunohistochemically the cellular distribution of COX-1 and COX-2 proteins in the human stomach with or without gastritis or ulceration. PATIENTS AND METHODS: Tissues were obtained by surgical resection of gastric ulcers associated with perforation (n = 9) or by biopsy from Helicobacter pylori positive patients with gastric ulcers (n = 45) and H pylori negative healthy subjects (n = 15). COX expression was detected by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), western blotting, and light and electron microscopic immunohistochemistry. RESULTS: COX-2 mRNA and protein were detected in gastric ulcer tissues but not in intact gastric mucosa. COX-1 mRNA and protein were detected in the intact mucosa. COX-2 immunostaining was exclusively localised in macrophages and fibroblasts between necrotic and granulation tissues of the ulcer bed. The percentage of COX-2 expressing cells was significantly higher in open than in closed ulcers, and in gastritis than in gastric mucosa without H pylori infection. COX-1 immunoreactivity localised in lamina propria mesenchymal cells was similar in various stages of ulcer disease and in intact gastric mucosa. Electron microscopic immunohistochemistry revealed both COX-1 and COX-2 on the luminal surfaces of the endoplasmic reticulum and nuclear envelope of macrophages and fibroblasts. CONCLUSIONS: Our results showed that COX-2 protein was induced in macrophages and fibroblasts in gastric ulcers and H pylori related gastritis, suggesting its involvement in the tissue repair process.
Authors:
A Tatsuguchi; C Sakamoto; K Wada; T Akamatsu; T Tsukui; K Miyake; S Futagami; T Kishida; Y Fukuda; N Yamanaka; M Kobayashi
Related Documents :
15645288 - Expression of homeodomain protein cdx2 in gallbladder carcinomas.
11566628 - Quantitation of chemokines (mdc, tarc) expression in mucosa from crohn's disease and ul...
11052998 - Novel goblet cell gene related to iggfcgammabp is regulated in adapting gut after small...
19351608 - Molecular characterisation and expression analysis of serex-defined antigen nucb2 in ga...
18234478 - Differential rna expression between schizophrenic patients and controls of the dystrobr...
15977188 - Effect of silybin and its glycosides on the expression of cytochromes p450 1a2 and 3a4 ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gut     Volume:  46     ISSN:  0017-5749     ISO Abbreviation:  Gut     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-01     Completed Date:  2000-08-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  782-9     Citation Subset:  AIM; IM    
Affiliation:
Third Department of Internal Medicine, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Cyclooxygenase 1
Cyclooxygenase 2
Female
Gastritis / enzymology*,  microbiology
Helicobacter Infections* / enzymology
Helicobacter pylori / enzymology*
Humans
Immunohistochemistry
Isoenzymes / metabolism*
Male
Membrane Proteins
Microscopy, Electron
Middle Aged
Prostaglandin-Endoperoxide Synthases / metabolism*
Reverse Transcriptase Polymerase Chain Reaction / methods
Stomach Ulcer / enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Membrane Proteins; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS1 protein, human; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Role of lipase in the regulation of postprandial gastric acid secretion and emptying of fat in human...
Next Document:  Microsatellite instability in patients with multiple primary cancers of the gastrointestinal tract.