Document Detail

Localisation of caveolin in mammary tissue depends on cell type.
MedLine Citation:
PMID:  17468894     Owner:  NLM     Status:  MEDLINE    
Caveolins, components of caveolae, are expressed in mammary tissue. In order to determine whether caveolins are present in different mammary cell types and whether their localisation depends on the physiological stage or species, cav-1 and cav-2 were characterised by immunoblotting in mammary tissues from the mouse, ewe and rabbit and localised, by immunofluorescence and electron microscopy, in mammary tissues from the mouse and ewe. At all the physiological stages studied, cav-1 and cav-2 were present in endothelial and myoepithelial cells in which flask-shaped caveolae were abundant. However, labelling of cav-1 and cav-2 associated with small vesiculo-tubular structures (including those close to lipid droplets) was low in epithelial cells. To study the possible association of cav-1 with lipid droplets, lactating ewe mammary fragments were treated in vitro with brefeldin A. This treatment did not modify the association of cav-1-labelled structures with lipid droplets. Finally, HC11 and MCF-10A mammary cell lines were treated with oleic acid. The total quantity of cav-1 was little affected by the treatment, although the lipid droplet labelling of cav-1 was amplified in MCF-10A cells. Thus, the synthesis and localisation of caveolins are mostly dependent upon the cell types of mammary tissue and upon their state of differentiation.
Catherine Hue-Beauvais; Christine Péchoux; Edwige Bouguyon; Sophie Chat; Sandrine Truchet; Alain Pauloin; Yann Le Gouar; Michèle Ollivier-Bousquet
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Publication Detail:
Type:  Journal Article     Date:  2007-02-14
Journal Detail:
Title:  Cell and tissue research     Volume:  328     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  521-36     Citation Subset:  IM    
Unité UR1196 Génomique et Physiologie de la Lactation, INRA, 78352, Jouy-en-Josas cedex, France.
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MeSH Terms
Caveolin 1 / analysis*
Caveolin 2 / analysis*
Cell Differentiation
Cells, Cultured
Lactation / metabolism
Mammary Glands, Animal / chemistry*,  cytology*,  growth & development
Mice, Inbred C57BL
Mice, Inbred CBA
Tissue Distribution
Reg. No./Substance:
0/Caveolin 1; 0/Caveolin 2

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