Document Detail


Localisation of caveolin in mammary tissue depends on cell type.
MedLine Citation:
PMID:  17468894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caveolins, components of caveolae, are expressed in mammary tissue. In order to determine whether caveolins are present in different mammary cell types and whether their localisation depends on the physiological stage or species, cav-1 and cav-2 were characterised by immunoblotting in mammary tissues from the mouse, ewe and rabbit and localised, by immunofluorescence and electron microscopy, in mammary tissues from the mouse and ewe. At all the physiological stages studied, cav-1 and cav-2 were present in endothelial and myoepithelial cells in which flask-shaped caveolae were abundant. However, labelling of cav-1 and cav-2 associated with small vesiculo-tubular structures (including those close to lipid droplets) was low in epithelial cells. To study the possible association of cav-1 with lipid droplets, lactating ewe mammary fragments were treated in vitro with brefeldin A. This treatment did not modify the association of cav-1-labelled structures with lipid droplets. Finally, HC11 and MCF-10A mammary cell lines were treated with oleic acid. The total quantity of cav-1 was little affected by the treatment, although the lipid droplet labelling of cav-1 was amplified in MCF-10A cells. Thus, the synthesis and localisation of caveolins are mostly dependent upon the cell types of mammary tissue and upon their state of differentiation.
Authors:
Catherine Hue-Beauvais; Christine Péchoux; Edwige Bouguyon; Sophie Chat; Sandrine Truchet; Alain Pauloin; Yann Le Gouar; Michèle Ollivier-Bousquet
Related Documents :
7357614 - Specificity of tissue interaction and origin of mesenchymal cells in the androgen respo...
1371414 - Differential roles of stromal cells, interleukin-7, and kit-ligand in the regulation of...
9538324 - Expression of parathyroid hormone-related protein (pthrp) and the pth/pthrp receptor in...
10598044 - 17beta-hydroxysteroid dehydrogenases in normal human mammary epithelial cells and breas...
18325704 - Electromagnetic field therapy delays cellular senescence and death by enhancement of th...
23072634 - The nontoxic cell cycle modulator indirubin augments transduction of adeno-associated v...
Publication Detail:
Type:  Journal Article     Date:  2007-02-14
Journal Detail:
Title:  Cell and tissue research     Volume:  328     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  521-36     Citation Subset:  IM    
Affiliation:
Unité UR1196 Génomique et Physiologie de la Lactation, INRA, 78352, Jouy-en-Josas cedex, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Caveolin 1 / analysis*
Caveolin 2 / analysis*
Cell Differentiation
Cells, Cultured
Female
Humans
Lactation / metabolism
Mammary Glands, Animal / chemistry*,  cytology*,  growth & development
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Pregnancy
Rabbits
Sheep
Tissue Distribution
Weaning
Chemical
Reg. No./Substance:
0/Caveolin 1; 0/Caveolin 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses.
Next Document:  VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.