| Local translation of dendritic RhoA revealed by an improved synaptoneurosome preparation. | |
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MedLine Citation:
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PMID: 20035871 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Changes in dendritic spine morphology, a hallmark of synaptic plasticity, involve remodeling of the actin cytoskeleton, a process that is regulated by Rho GTPases. RhoA, a member of this GTPase family, segregates to dendrites in differentiated neurons. Given the emerging role of dendritic mRNA local translation in synaptic plasticity, we have assessed the possible localization and translation of RhoA mRNA at dendrites. At this end, we have developed and describe here in detail an improved method for isolating hippocampal and neocortical mouse synaptoneurosomes. This synaptoneurosomal preparation is much more enriched in synaptic proteins than those obtained in former methods, exhibits bona fide electron microscopy pre- and postsynaptic morphologies, contains abundant dendritic mRNAs, and is competent for activity-regulated protein synthesis. Using this preparation, we have found that RhoA mRNA is dendritically localized and its local translation is enhanced by BDNF stimulation. These findings suggest that some of the known functions of RhoA on spine morphology may be mediated by regulating its local translation. |
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Authors:
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Jos? A Troca-Mar?n; Alexandra Alves-Sampaio; Francisco J Tejedor; Mar?a Luz Montesinos |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-24 |
Journal Detail:
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Title: Molecular and cellular neurosciences Volume: 43 ISSN: 1095-9327 ISO Abbreviation: Mol. Cell. Neurosci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-05-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100095 Medline TA: Mol Cell Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 308-14 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Departamento de Fisiolog?a M?dica y Biof?sica, Universidad de Sevilla, E-41009 Sevilla, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain-Derived Neurotrophic Factor / metabolism Cytoskeleton / metabolism Dendrites / metabolism*, ultrastructure Hippocampus / cytology, metabolism Mice Mice, Inbred C57BL Neuronal Plasticity / physiology Neurons* / cytology, metabolism Protein Biosynthesis Synaptosomes / chemistry*, metabolism rhoA GTP-Binding Protein / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Brain-Derived Neurotrophic Factor; EC 3.6.5.2/rhoA GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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