| Local suppression of pro-inflammatory cytokines and the effects in BMP-2-induced bone regeneration. | |
| | |
MedLine Citation:
|
PMID: 22019155 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The objective of this study is to investigate the effect of local inflammation suppression on the bone regeneration. Gelatin hydrogels incorporating mixed immunosuppressive triptolide-micelles and bone morphogenic protein-2 (BMP-2) were prepared. The controlled release of both the triptolide and BMP-2 from the hydrogels was observed under in vitro and in vivo conditions. When either J774.1 macrophage-like or MC3T3-E1 osteoblastic cells were cultured in the hydrogels incorporating mixed 2.5, 5 or 10 mg of triptolide-micelles and BMP-2, the expression level of pro- and anti-inflammatory cytokines including interleukin (IL)-6 and IL-10 was down-regulated, but the alkaline phosphatase (ALP) activity was promoted compared with those of hydrogels incorporating BMP-2 without triptolide-micelles. When implanted into a critical-sized bone defect of rats, the hydrogels incorporating mixed 2.5 or 5 mg of triptolide-micelles and BMP-2 showed significantly lower number of neutrophils, lymphocytes, macrophages or dendritic and mast cells infiltrated into the defect, and lower expression level of IL-6, TNF-α, and IL-10 than those incorporating BMP-2 without triptolide-micelles. The reduced local inflammation responses at the defects implanted with the hydrogels incorporating mixed 2.5 or 5 mg of triptolide-micelles and BMP-2 subsequently enhanced the bone regeneration thereat. It is concluded that the proper local modulation of inflammation responses is a promising way to achieve the enhanced bone regeneration. |
| | |
Authors:
|
Juthamas Ratanavaraporn; Hiroyuki Furuya; Yasuhiko Tabata |
Related Documents
:
|
19996195 - Strong purified protein derivative responses are associated with poor mycobacterium inh... 7960155 - Bacterial heat shock proteins directly induce cytokine mrna and interleukin-1 secretion... 21704385 - Interaction between interleukin-1 receptor 2 and toll-like receptor 4, and cervical cyt... 21796075 - Effects of lipoic acid, caffeic acid and a synthesized lipoyl-caffeic conjugate on huma... 20400705 - St2 negatively regulates tlr2 signaling, but is not required for bacterial lipoprotein-... 3159805 - Human hypersensitivity angiitis, an immune complex disease. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-20 |
Journal Detail:
|
Title: Biomaterials Volume: - ISSN: 1878-5905 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Laryngeal abnormalities are frequent in the 22q11 deletion syndrome.
Next Document: Observations on Hydroceles Following Adolescent Varicocelectomy.