| Local lymphogenic migration pathway in normal mouse spleen. | |
| | |
MedLine Citation:
|
PMID: 19898873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although the immunological and hemodynamical significance of the spleen is of great importance, few reports detail the lymphatic vessels in this organ. We have used an immunohistochemical three-dimensional imaging technique to characterize lymphatic vessels in the normal mouse spleen and have successfully demonstrated their spatial relationship to the blood vascular system for the first time. Lymphatic markers, such as LYVE-1, VEGFR-3, and podoplanin, show different staining patterns depending on their location in the spleen. LYVE-1-positive lymphatic vessels run reverse to the arterial blood flow along the central arteries in the white pulp and trabecular arteries and exit the spleen from the hilum. These lymphatic vessels are surrounded by type IV collagen, indicating that they are collecting lymphatic vessels rather than lymphatic capillaries. Podoplanin is expressed not only in lymphatic vessels, but also in stromal cells in the white pulp. These podoplanin-positive cells form fine meshworks surrounding the lymphatic vessels and central arteries. Following intravenous transplantation of lymphocytes positive for green fluorescent protein (GFP(+)) into normal recipient mice, donor cells appear in the meshworks within 1 h and accumulate in the lymphatic vessels within 6 h after injection. The GFP(+) cells further accumulate in a draining celiac lymph node through the efferent lymphatic vessels from the hilum. These meshworks might therefore act as an extravascular lymphatic pathway and, together with ordinary lymphatic vessels, play a primary role in the cell traffic of the spleen, additional to the blood circulatory system. |
| | |
Authors:
|
Kazuhiko Shimizu; Shunichi Morikawa; Shuji Kitahara; Taichi Ezaki |
Related Documents
:
|
3957833 - Contamination of lung lymph following standard and modified procedures in sheep. 7735643 - A mathematical model of flow through the terminal lymphatics. 16041793 - Biomechanical properties of different segments of human umbilical cord vein and its val... |
Publication Detail:
|
Type: Journal Article Date: 2009-11-07 |
Journal Detail:
|
Title: Cell and tissue research Volume: 338 ISSN: 1432-0878 ISO Abbreviation: Cell Tissue Res. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2010-01-21 Completed Date: 2010-04-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
|
Languages: eng Pagination: 423-32 Citation Subset: IM |
Affiliation:
|
Department of Anatomy and Developmental Biology, School of Medicine, Tokyo Women's Medical University, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Vessels / ultrastructure Chemokine CCL21 / analysis Endothelial Cells / cytology* Female Green Fluorescent Proteins / analysis Lymphatic Vessels / ultrastructure* Male Membrane Glycoproteins / analysis Mice Mice, Inbred C57BL Spleen / ultrastructure* |
| Chemical | |
Reg. No./Substance:
|
0/Chemokine CCL21; 0/Gp38 protein, mouse; 0/Membrane Glycoproteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nanoscale engineering of biomimetic surfaces: cues from the extracellular matrix.
Next Document: Cardiovascular risk in chronic kidney disease (CKD): the CKD-mineral bone disorder (CKD-MBD).