| Local heat shock priming promotes recanalization of thromboembolized microvasculature by upregulation of plasminogen activators. | |
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MedLine Citation:
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PMID: 16627801 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Thromboembolization and subsequent microvascular perfusion failure is implicated in the pathology of a variety of diseases, including transient ischemic attack (TIA), stroke, and myocardial infarction, and also for the complications after interventional and microsurgical procedures in coronary heart disease and peripheral arterial occlusive disease. In vitro heat shock priming has been suggested to induce plasminogen activators, which are the major upregulators of the fibrinolytic system. Herein, we determined whether local heat shock priming endogenously upregulates plasminogen activators also in vivo, and whether this promotes recanalization of thromboembolized microvasculature. METHODS AND RESULTS: To induce thromboembolization, a suspension of preformed microthrombi (maximum diameter: 40 microm) was injected via the femoral artery into the left hindlimbs of anesthetized rats. Local heat shock priming (42.5 degrees C, 30 minutes) was performed 24 hours before embolization and resulted in a significant increase of endothelium-derived plasminogen activator expression. The study of the microcirculation by intravital microscopy revealed in all tissues analyzed (muscle, periosteum, subcutis, and skin) that heat shock priming significantly (P<0.05) accelerates recanalization of the thromboembolized microvasculature when compared with nonprimed and sham-primed controls. Importantly, the addition of plasminogen activator inhibitor-1 to the microthrombi suspension completely blunted the heat shock-induced acceleration of microvascular recanalization. CONCLUSIONS: Heat shock induces endogenous hyperfibrinolysis by upregulation of plasminogen activators that promote recanalization of thromboembolized microvasculature. |
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Authors:
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Martin Rücker; Thilo Schäfer; Claudia Scheuer; Yves Harder; Brigitte Vollmar; Michael D Menger |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-20 |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 26 ISSN: 1524-4636 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-23 Completed Date: 2006-07-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1632-9 Citation Subset: IM |
Affiliation:
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Institute for Clinical & Experimental Surgery, University of Saarland, D-66421 Homburg/Saar, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fibrinolysis* Heat-Shock Response* Microcirculation Plasminogen Activator Inhibitor 1 / blood Plasminogen Activators / metabolism* Rats Rats, Sprague-Dawley Thromboembolism / physiopathology* Tissue Plasminogen Activator / blood Up-Regulation* Urokinase-Type Plasminogen Activator / blood |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Activator Inhibitor 1; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.68/Tissue Plasminogen Activator; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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