Document Detail

Local effect of IL-4 delivery on polyethylene particle induced osteolysis in the murine calvarium.
MedLine Citation:
PMID:  23225668     Owner:  NLM     Status:  Publisher    
Wear particles generated with use of total joint replacements incite a chronic macrophage-mediated inflammatory reaction, which leads to implant failure. Macrophage activation may be polarized into two states, with an M1 proinflammatory state dominating an alternatively activated M2 anti-inflammatory state. We hypothesized that IL-4, an activator of M2 macrophages, could modulate polyethylene (PE) particle-induced osteolysis in an experimental murine model. Four animal groups included (a) calvarial saline injection with harvest at 14 days (b) single calvarial injection of PE particles subcutaneously (SC) without IL-4 (c) PE particles placed as in (b), then IL-4 given SC for 14 consecutive days and (d) PE particles as in (b) then IL-4 beginning 7 days after particle injection for 7 days. The calvarial bone volume to total tissue volume was measured using microCT and histomorphometry. Calvaria were cultured for 24 h to assess release of RANKL, OPG, TNF-α, and IL-1ra and isolation and identification of M1 and M2 specific proteins. MicroCT and histomorphometric analysis showed that bone loss was significantly decreased following IL-4 administration to PE treated calvaria for both 7 and 14 days. Western blot analysis showed an increased M1/M2 ratio in the PE treated calvaria, which decreased with addition of IL-4. Cytokine analysis showed that the RANKL/OPG ratio and TNF-α/IL-1ra ratio decreased in PE-treated calvaria following IL-4 addition for 14 days. IL-4 delivery mitigated PE particle-induced osteolysis through macrophage polarization. Modulation of macrophage polarization is a potential treatment strategy for wear particle induced periprosthetic osteolysis. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Allison J Rao; Christophe Nich; Lakshmi S Dhulipala; Emmanuel Gibon; Roberto Valladares; Stefan Zwingenberger; R Lane Smith; Stuart B Goodman
Related Documents :
22969768 - Transcriptional regulation of the anti-inflammatory cytokine il-10 in acquired immune c...
23092328 - Heme oxygenase-1 and breast cancer resistance protein protect against heme-induced toxi...
23965728 - Vitexin 6, a novel lignan, induces autophagy and apoptosis by activating the jun n-term...
24123678 - Targeting f box protein fbxo3 to control cytokine-driven inflammation.
20060598 - Sphingomyelinase dependent apoptosis of dendritic cells following treatment with amyloi...
16988278 - Escherichia coli prevents phagocytosis-induced death of macrophages via classical nf-ka...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Orthopaedic Surgery, Orthopaedic Research Laboratories, Stanford University School of Medicine, Edwards R116, Stanford, California 94305.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A Three-Dimensional Branched Cobalt-Doped ?-Fe(2) O(3) Nanorod/MgFe(2) O(4) Heterojunction Array as ...
Next Document:  Integration-Free Induced Pluripotent Stem Cells Model Genetic and Neural Developmental Features of D...