| Local delivery of c-myb antisense oligonucleotides during balloon angioplasty. | |
| | |
MedLine Citation:
|
PMID: 9213020 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Intraluminal delivery of antisense oligonucleotides to c-myb was assessed following balloon angioplasty in swine peripheral arteries. Successful delivery and intramural persistence of oligonucleotide for over 24 h were demonstrated following angioplasty with hydrogel balloons coated with 32P-labeled antisense. Delivery of fluorescein-labeled antisense demonstrated further localization within the arterial media and intracellularly. Preliminary in vitro studies demonstrated the feasibility of inhibition of porcine lymphocyte proliferation using the murine antisense to c-myb. Twelve iliac or carotid arteries underwent angioplasty with antisense-coated balloons, while the contralateral vessels underwent angioplasty with the same-sized balloons coated with the complementary sense strand. Six to seven days later, dilated arterial segments were surgically isolated. In 10 of 12 vessel pairs, antisense-treated vessels demonstrated less cellular proliferation than did contralateral sense-treated vessels, as assessed by quantitative immunohistochemical staining of proliferating cell nuclear antigen, and smooth muscle cell proliferation was reduced 18% in antisense-treated vessels compared to the contralateral sense-treated vessels (PCNA-positive nuclear area: 7.7 +/- 4.9% vs. 9.3 +/- 5.2%, P < 0.04)-intraluminal delivery of antisense oligonucleotides to c-myb is feasible with a catheter-based system and may reduce smooth muscle cell proliferation following arterial injury. |
| | |
Authors:
|
M A Azrin; J F Mitchel; L M Bow; C A Pedersen; R W Cartun; T H Aretz; D D Waters; R G McKay |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Catheterization and cardiovascular diagnosis Volume: 41 ISSN: 0098-6569 ISO Abbreviation: Cathet Cardiovasc Diagn Publication Date: 1997 Jul |
Date Detail:
|
Created Date: 1997-08-21 Completed Date: 1997-08-21 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7508512 Medline TA: Cathet Cardiovasc Diagn Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 232-40 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine and Cardiology, Hartford Hospital, Connecticut, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angioplasty, Transluminal, Percutaneous Coronary
/
instrumentation* Animals Autoradiography Cell Division / drug effects Coronary Disease / drug therapy*, pathology Coronary Vessels / drug effects, pathology Drug Delivery Systems / instrumentation* Feasibility Studies Female Lymphocyte Activation / drug effects Mice Microscopy, Fluorescence Muscle, Smooth, Vascular / drug effects, pathology Oligonucleotides, Antisense / administration & dosage*, pharmacokinetics Proliferating Cell Nuclear Antigen / metabolism Proto-Oncogene Proteins / antagonists & inhibitors*, metabolism Proto-Oncogene Proteins c-myb Swine Trans-Activators / antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Oligonucleotides, Antisense; 0/Proliferating Cell Nuclear Antigen; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/Trans-Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tibial ultrasound velocity measured in situ predicts the material properties of tibial cortical bone...
Next Document: Feasibility and efficacy of locally delivered enoxaparin via the Channeled Balloon catheter on smoot...