Document Detail

Local cell proliferation in rheumatoid synovial tissue: analysis by cyclin expression.
MedLine Citation:
PMID:  16518573     Owner:  NLM     Status:  MEDLINE    
The immunohistochemical staining of cyclins was done to evaluate the proliferating cells in synovial tissue of rheumatoid arthritis (RA). Synovial specimens obtained from 18 patients with RA, 12 with osteoarthritis (OA), and 8 with traumatic arthritis (TA) were used for immunostaining of cyclins A and B1 and proliferating cell nuclear antigen (PCNA). The positive cells in lining layer (synoviocytes) and sublining layer (lymphoid and nonlymphoid cells) were counted. Moreover, the relationship between the frequency of their positive cells and clinical data of RA patients was analyzed statistically. In general, cyclin-A-, cyclin-B1-, and PCNA-positive cells in RA were more frequently observed as compared with those in OA and TA. Significant differences were found between RA and OA or TA in cyclin-A-, cyclin-B1-, and PCNA-positive sublining lymphoid cells, between RA and OA or TA in cyclin-B1- and PCNA-positive sublining nonlymphoid cells, and between RA and OA in cyclin-B1-positive synoviocytes. The ratio of cyclin-A- or cyclin-B1-positive cells per PCNA-positive cells was significantly higher in sublining lymphoid cells in RA than TA and in sublining lymphoid and nonlymphoid cells of RA than OA or TA. Moreover, a better relationship was observed between the frequency of cyclin-A-positive synoviocytes and age and between cyclin-A-positive sublining nonlymphoid cells and duration of the disease in RA patients. Our data demonstrated clearly that synoviocytes, as well as sublining lymphoid and nonlymphoid cells, could divide in situ, and more frequent cell division and a higher ratio of cyclin-A- or cyclin-B1-positive/PCNA-positive sublining cells could occur in RA than OA and TA.
Chikako Takahashi Tohyama; Mitsunori Yamakawa; Akira Murasawa; Kiyoshi Nakazono; Hajime Ishikawa
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Publication Detail:
Type:  Journal Article     Date:  2006-03-02
Journal Detail:
Title:  Clinical rheumatology     Volume:  25     ISSN:  0770-3198     ISO Abbreviation:  Clin. Rheumatol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-09-26     Completed Date:  2007-01-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8211469     Medline TA:  Clin Rheumatol     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  801-6     Citation Subset:  IM    
Department of Orthopedic Surgery, Rheumatic Center, Niigata Prefectural Senami Hospital, 2-4-15, Senami-Onsen, Murakami, 958-8555, Niigata, Japan.
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MeSH Terms
Aged, 80 and over
Aging / metabolism
Arthritis / etiology,  metabolism,  pathology
Arthritis, Rheumatoid / metabolism*,  pathology*
Cell Proliferation*
Cyclin A / metabolism
Cyclin B / metabolism
Cyclin B1
Cyclins / metabolism*
Immunohistochemistry / methods
Joints / injuries
Middle Aged
Osteoarthritis / metabolism,  pathology
Proliferating Cell Nuclear Antigen / metabolism
Staining and Labeling
Synovial Membrane / metabolism*,  pathology*
Time Factors
Wounds and Injuries / complications
Reg. No./Substance:
0/CCNB1 protein, human; 0/Cyclin A; 0/Cyclin B; 0/Cyclin B1; 0/Cyclins; 0/Proliferating Cell Nuclear Antigen

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