| Local blood flow regulation in transplanted rat pancreatic islets: influence of adenosine, angiotensin II, and nitric oxide inhibition. | |
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MedLine Citation:
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PMID: 10933149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Transplanted islets lack endothelial cells immediately after implantation and therefore depend on an adequate revascularization for their survival and function. However, the functional properties of the newly formed islet graft microvessels are largely unknown. This study aimed to investigate the blood flow regulation of transplanted pancreatic islets. METHODS: Pancreatic islets were syngeneically transplanted beneath the renal capsule of control and streptozotocin-diabetic rats. Blood flow measurements were performed 4 weeks later using laser-Doppler flowmetry. Adenosine (0.6 mg x kg(-1) x min(-1), angiotensin II (AT II; 0.17 microg x kg(-1) x min(-1)) and the nitric oxide synthase inhibitor NG-nitro-L-arginine (25 mg/ kg) were given to each animal. RESULTS: An increased basal blood flow and basal vascular conductance in the islet grafts, but not in the renal cortex, were seen in diabetic rats compared with control rats. Adenosine increased, and AT II decreased, the vascular conductance of the islet grafts in both nondiabetic and diabetic animals. A more pronounced circulatory response to AT II was observed in kidneys of diabetic animals, whereas there was no difference in the islet graft blood flow response between nondiabetic and diabetic animals. NG-Nitro-L-arginine decreased islet graft blood flow and vascular conductance in both nondiabetic and diabetic recipients, but the effect was more pronounced in the non-diabetic animals. CONCLUSIONS: Islet graft blood flow was influenced by adenosine, AT II, and nitric oxide inhibition in all animals. However, diabetic animals were less dependent on nitric oxide to maintain a basal blood flow in the islet graft. |
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Authors:
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R Olsson; L Jansson; A Andersson; P O Carlsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation Volume: 70 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-08-24 Completed Date: 2000-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 280-7 Citation Subset: IM |
Affiliation:
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Department of Medical Cell Biology, Uppsala University, Sweden. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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pharmacology Angiotensin II / pharmacology Animals Blood Glucose / metabolism Blood Pressure Body Weight Enzyme Inhibitors / pharmacology Hematocrit Hyperglycemia / metabolism Infusions, Intravenous Injections Insulin / blood Islets of Langerhans / blood supply Islets of Langerhans Transplantation / physiology* Male Nitric Oxide Synthase / antagonists & inhibitors Nitroarginine / pharmacology Rats Rats, Inbred WF Regional Blood Flow / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Enzyme Inhibitors; 11061-68-0/Insulin; 11128-99-7/Angiotensin II; 2149-70-4/Nitroarginine; 58-61-7/Adenosine; EC 1.14.13.39/Nitric Oxide Synthase |
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