Document Detail

Local antibody response in Peyer's patches to the orally administered dietary protein antigen.
MedLine Citation:
PMID:  10664845     Owner:  NLM     Status:  MEDLINE    
To understand local antibody production to dietary protein antigens in the gut, the reactivity of the monoclonal antibodies (mAbs) from Peyer's patches of BALB/c mice raised against orally administered hen egg lysozyme (HEL) was studied. These mAbs were of IgG1 (7 clones), IgA (5 clones) and IgM (13 clones) isotypes. Some of the HEL-binding mAbs preferentially reacted with reduced, carboxy-methylated HEL, rather than with native HEL. MAbs of the IgA and IgM isotypes had cross-reactivity with other unrelated environmental antigens such as E. coli, single-strand DNA, and soluble components of mouse food. In contrast, the IgG1 mAbs did not cross-react with these antigens. The average of the Kd values for HEL of these mAbs was in the order of 10(-6) M, which is moderately higher than those of mAbs from the preimmune repertoire. These results suggest that, under normal physiological conditions, orally administered dietary proteins predominantly induce the local production of polyreactive IgA/IgM antibodies cross-reacting with environmental luminal antigens.
M Shimoda; Y Inoue; N Azuma; C Kanno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  63     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-03-21     Completed Date:  2000-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  2123-9     Citation Subset:  IM    
Department of Applied Biochemistry, Utsunomiya University, Tochigi, Japan.
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MeSH Terms
Administration, Oral
Antibodies, Monoclonal
Antibody Formation
Dietary Proteins / immunology*
Enzyme-Linked Immunosorbent Assay
Food Hypersensitivity / immunology*
Hybridomas / immunology,  metabolism
Immunoglobulin A / metabolism
Immunoglobulin G / metabolism
Immunoglobulin M / metabolism
Mice, Inbred BALB C
Peyer's Patches / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Dietary Proteins; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M

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