Document Detail


Local administration of uridine suppresses the cardinal features of asthmatic airway inflammation.
MedLine Citation:
PMID:  20455899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The immuno-modulatory properties of nucleotides such as adenosine or inosine, have been described extensively. Recently, the nucleoside uridine and its analogue 4-thiouridine have gained attention for their protective role in acute lung inflammation.
OBJECTIVE: In this study, we investigated the influence of uridine on asthmatic airway inflammation.
METHODS: We used the classical ovalbumin (OVA)-alum model, as well as a model of house dust mite-(HDM)-induced airway inflammation. The degree of inflammation was determined by bronchoalveolar lavage (BAL), histology, and measurement of bronchial hyperresponsiveness.
RESULTS: Intratracheal treatment of OVA-sensitized animals with uridine before allergen challenge resulted in a reduction in total BAL cells and BAL eosinophils. This was accompanied by reduced tissue infiltration and diminished production of T helper type 2-cytokines by mediastinal lymph node cells. Additionally, mice treated with uridine developed less bronchial hyperresponsiveness. Uridine was also effective in reducing airway inflammation in HDM-induced asthma. The protective effects of uridine were independent of myeloid dendritic cell (mDC) function, because in vitro pre-treatment of allergen-pulsed DCs with uridine did not alter the degree of inflammation. However, uridine inhibited the release of pro-inflammatory mediators in vivo and by cultured lung epithelial cells, suggesting an effect on lung structural cells.
CONCLUSION: In summary, we were able to show that uridine inhibits the classical features of asthmatic airway inflammation. As uridine supplementation is well tolerated in humans, it might be a new therapeutic approach for the treatment of bronchial asthma.
Authors:
T Müller; M Grimm; R P de Vieira; S Cicko; T Dürk; S Sorichter; G Zissel; M Idzko
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  40     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-12     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  1552-60     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Affiliation:
Department of Pneumology, University Medical Hospital Freiburg, Freiburg, Germany. tobias.mueller@uniklinikfreiburg.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents / pharmacology*
Asthma / drug therapy*,  immunology
Bronchoalveolar Lavage Fluid / chemistry,  immunology
Cell Separation
Cytokines / biosynthesis
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Inflammation / drug therapy,  immunology
Mice
Mice, Inbred BALB C
Ovalbumin / immunology
Pneumonia / drug therapy*,  immunology
Uridine / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Cytokines; 58-96-8/Uridine; 9006-59-1/Ovalbumin
Comments/Corrections
Comment In:
Clin Exp Allergy. 2010 Oct;40(10):1436-8   [PMID:  20937059 ]

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