| Local administration of NF-kappa B decoy oligonucleotides to prevent restenosis after balloon angioplasty: an experimental study in New Zealand white rabbits. | |
| | |
MedLine Citation:
|
PMID: 15886949 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To evaluate the efficacy of NF-kappa B oligonucleotides (ODN) administered by local administration with the channeled balloon catheter to prevent restenosis after balloon angioplasty in restenotic iliac arteries of New Zealand white rabbits. MATERIALS AND METHODS: In vitro, 8000 rabbit vascular smooth muscle cells (rVSMC) where transfected with a liposomal carrier (TfX50) with 100 ng of decoy and scrambled ODN. Inhibition of proliferation was measured using a MTT assay after 24 hours in comparison to control. In vivo, 22 male New Zealand White rabbits were fed a 1% cholesterol diet and received denudation of both common iliac arteries with a 3 mm balloon catheter to induce an arterial stenosis. Four weeks after stenosis induction, local application of NF-kappa B in two different concentrations (1 mug: n = 14; 10 mug: n = 8) was performed randomly on one common iliac artery. Scrambled oligonucleotides without specific binding capacities were injected into the contralateral side. The channeled balloon catheter allows simultaneous balloon dilation (8 atm) of the stenosis and local application of a drug solution (2 atm). Four weeks after local drug delivery the animals were killed and the vessels were excised and computerized morphometric measurements were performed. RESULTS: NF-kappa B decoy ODN but not scrambled ODN inhibited proliferation of rVSMC in vitro. Following local ODN application in the animals, no acute vascular complications were seen. NF-kappa B ODN resulted in a statistically non significant reduction of neointimal area compared to the control group. The neointimal area was 0.97 mm(2) using 1 mug NF-kappa B ODN compared to 0.98 mm(2) in the control group. The higher dose resulted in a neointimal area of 0.97 mm(2) compared to 1.07 mm(2) at the control side. CONCLUSIONS: Local drug delivery of NF-kappa B ODN using the "channeled balloon" catheter could not reduce neointimal hyperplasia in stenostic rabbit iliac arteries. Application modalities have to be improved to enhance the effect of the local application to prevent restenosis after balloon angioplasty. |
| | |
Authors:
|
Marc Kalinowski; Kerstin Viehofer; Christine Hamann; James J Barry; Beate Kleb; Klaus Jochen Klose; Hans-Joachim Wagner; Heiko Alfke |
Related Documents
:
|
451879 - Fibromuscular dysplasia of the external iliac arteries: surgical treatment by graduated... 10148089 - Interventional catheterization in congenital heart disease. 487419 - Massive hemoptysis secondary to flow-directed thermodilution catheters. 1130899 - The use of vein allografts as sidearms for intraaortic balloon insertion. 12124969 - Patterns of late diameter change after balloon angioplasty of branch pulmonary artery s... 2933199 - Balloon pump support of the failing right heart. 16981919 - Mechanical esophageal deflection during ablation of atrial fibrillation. 7049289 - Pharmacological responses of human and porcine lung parenchyma, bronchus and pulmonary ... 10914599 - Unexpected sudden death from coronary sinus thrombosis. an unusual complication of cent... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Cardiovascular and interventional radiology Volume: 28 ISSN: 0174-1551 ISO Abbreviation: Cardiovasc Intervent Radiol Publication Date: 2005 May-Jun |
Date Detail:
|
Created Date: 2005-06-03 Completed Date: 2005-09-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8003538 Medline TA: Cardiovasc Intervent Radiol Country: United States |
Other Details:
|
Languages: eng Pagination: 331-7 Citation Subset: IM |
Affiliation:
|
Department of Diagnostic Radiology, Philipps University Hospital, Baldingerstrasse, 35033 Marburg, Germany. kalinows@mailer.uni-marburg.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angioplasty, Balloon* Animals Arterial Occlusive Diseases / pathology, prevention & control*, therapy Balloon Dilatation* / instrumentation Cell Proliferation / drug effects Cells, Cultured Constriction, Pathologic / pathology, prevention & control Drug Delivery Systems Hyperplasia Iliac Artery / drug effects*, pathology Male Muscle, Smooth, Vascular / drug effects, pathology Oligodeoxyribonucleotides / administration & dosage* Rabbits Random Allocation Tunica Intima / drug effects, pathology |
| Chemical | |
Reg. No./Substance:
|
0/NF-kappaB decoy; 0/Oligodeoxyribonucleotides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Predicting infected bile among patients undergoing percutaneous cholecystostomy.
Next Document: Calculating colon transit time with radionuclide-filled capsules in constipated patients: a new meth...