| Local adenoviral-mediated expression of recombinant hirudin reduces neointima formation after arterial injury. | |
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MedLine Citation:
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PMID: 8612227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Catalytically active thrombin, acting locally, is thought to mediate neointima formation after arterial injury. We constructed an adenovirus vector, AdHV-1.2, containing a complementary DNA for the thrombin inhibitor hirudin. AdHV-1.2 directed the synthesis and secretion of biologically active hirudin from vascular cells in vitro. In vivo gene transfer of hirudin into smooth muscle cells of injured rat carotid arteries resulted in peak secretion of at least 34+/-23 pg hirudin per vessel per 24 hours, and resulted in a significant (P<0.05) 35% reduction in neointima formation. Systemic partial thromboplastin times were not affected by local hirudin expression. These results support the hypothesis that local thrombin activity contributes to neointima formation after arterial injury and suggest that local delivery of a highly specific antithrombin may constitute an effective intervention for arterial proliferative disease. |
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Authors:
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J J Rade; A H Schulick; R Virmani; D A Dichek |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nature medicine Volume: 2 ISSN: 1078-8956 ISO Abbreviation: Nat. Med. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-06-05 Completed Date: 1996-06-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9502015 Medline TA: Nat Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 293-8 Citation Subset: IM |
Affiliation:
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Molecular Hematology Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Animals Base Sequence Carotid Artery Injuries* DNA, Complementary / genetics Gene Expression* Gene Transfer Techniques Genetic Vectors Hirudins / genetics*, metabolism Male Molecular Sequence Data Muscle, Smooth, Vascular / metabolism Neovascularization, Pathologic / prevention & control* Rats Rats, Sprague-Dawley Recombinant Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Hirudins; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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