Document Detail

Local NSAID infusion does not affect protein synthesis and gene expression in human muscle after eccentric exercise.
MedLine Citation:
PMID:  20738823     Owner:  NLM     Status:  In-Data-Review    
Unaccustomed exercise leads to satellite cell proliferation and increased skeletal muscle protein turnover. Several growth factors and cytokines may be involved in the adaptive responses. Non-steroidal anti-inflammatory drugs (NSAIDs) negatively affect muscle regeneration and adaptation in animal models, and inhibit the exercise-induced satellite cell proliferation and protein synthesis in humans. However, the cellular mechanisms eliciting these responses remain unknown. Eight healthy male volunteers performed 200 maximal eccentric contractions with each leg. To block prostaglandin synthesis locally in the skeletal muscle, indomethacin (NSAID) was infused for 7.5 h via microdialysis catheters into m. vastus lateralis of one leg. Protein synthesis was determined by the incorporation of 1,2-(13) C(2) leucine into muscle protein from 24 to 28 h post-exercise. Furthermore, mRNA expression of selected genes was measured in muscle biopsies (5 h and 8 days post-exercise) by real-time reverse transcriptase PCR. Myofibrillar and collagen protein synthesis were unaffected by the local NSAID infusion. Five hours post-exercise, the mRNA expression of cyclooxygenase-2 (COX2) was sixfold higher in the NSAID leg (P=0.016) compared with the unblocked leg. The expression of growth factors and matrix-related genes were unaffected by NSAID. Although NSAIDs inhibit the exercise-induced satellite cell proliferation, we observed only limited effects on gene expression, and on post-exercise protein synthesis.
U R Mikkelsen; P Schjerling; I C Helmark; S Reitelseder; L Holm; D Skovgaard; H Langberg; M Kjaer; K M Heinemeier
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Publication Detail:
Type:  Journal Article     Date:  2010-08-24
Journal Detail:
Title:  Scandinavian journal of medicine & science in sports     Volume:  21     ISSN:  1600-0838     ISO Abbreviation:  Scand J Med Sci Sports     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111504     Medline TA:  Scand J Med Sci Sports     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  630-44     Citation Subset:  IM    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Institute of Sports Medicine, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
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