Document Detail


Loading of myoglobin into multilayer films assembled by ZrO2 nanoparticles and phytic acid: electrochemistry and electrocatalysis.
MedLine Citation:
PMID:  19437988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Small-molecular phytic acid (PA) with its unique structure was successfully assembled with ZrO2 nanoparticles into {PA/ZrO2}n layer-by-layer films on solid surfaces, which was confirmed by quartz crystal microbalance (QCM) and cyclic voltammetry (CV) with K3Fe(CN)6 as the electroactive probe. Myoglobin (Mb) could be gradually "absorbed" or loaded into the films when the films were immersed into Mb solutions. The Mb-loaded films at pyrolytic graphite (PG) electrodes, designated as {PA/ZrO2}n-Mb, demonstrated well-defined and quasi-reversible CV responses for Mb Fe(III)/Fe(II) redox couple and good electrocatalytic properties toward oxygen and H2O2. The driving force of the film assembly and the interaction between Mb and {PA/ZrO2}n films were explored and discussed in detail. The coordination interaction between PA and ZrO2 is believed to be the main driving force for the assembly of {PA/ZrO2}n multilayer films, and the electrostatic attraction between oppositely charged Mb and the film components is the main interaction for Mb loading into the films. The loading behavior of the {PA/ZrO2}n films toward different proteins with different size indicates that while the porosity of the films is necessary for the protein loading, only those proteins with the size smaller than the average pore size of the films can be incorporated in the interior of films.
Authors:
Lingzhu Yang; Hongyun Liu; Naifei Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nanoscience and nanotechnology     Volume:  9     ISSN:  1533-4880     ISO Abbreviation:  J Nanosci Nanotechnol     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-05-14     Completed Date:  2009-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088195     Medline TA:  J Nanosci Nanotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2442-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Beijing Normal University, Beijing, 100875, China.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*
Catalysis
Electrochemistry
Electrodes
Graphite / chemistry
Hydrogen Peroxide / analysis,  metabolism
Immobilized Proteins / chemistry,  metabolism*
Myoglobin / chemistry,  metabolism*
Nanoparticles / chemistry*,  ultrastructure
Oxidation-Reduction
Oxygen / analysis,  metabolism
Phytic Acid / chemistry*
Zirconium / chemistry*
Chemical
Reg. No./Substance:
0/Immobilized Proteins; 0/Myoglobin; 1314-23-4/zirconium oxide; 7440-67-7/Zirconium; 7722-84-1/Hydrogen Peroxide; 7782-42-5/Graphite; 7782-44-7/Oxygen; 83-86-3/Phytic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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