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Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance.
MedLine Citation:
PMID:  21835877     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Listeria monocytogenes is a foodborne pathogen causing listeriosis. Acid is one of the most frequently stresses that foodborne pathogens encounter. The ability to survive and proliferate in acidic environments is a prerequisite for its infection. However, there is limited knowledge about the molecular basis of adaption of L. monocytogenes to acid. Arginine deiminase (ADI) and agmatine deiminase (AgDI) systems are implicated in bacterial tolerance to acidic environments. Homologues of ADI and AgDI systems were found in L. monocytogenes lineages I and II strains. Sequence analysis indicated that lmo0036 encoded a putative carbamoyltransferase containing conserved motifs and residues important for substrate binding. Lmo0036 acted as ornithine carbamoyltransferase and putrescine carbamoyltransferase, representing the first example that catalyzes reversible ornithine and putrescine carbamoyltransfer reactions. Catabolic ornithine and putrescine carbamoyltransfer reactions constituted the second step of ADI and AgDI pathways. However, the equilibrium of in vitro carbamoyltransfer reactions were overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase was probably the limiting step of the pathways. lmo0036 was induced at the transcriptional level when L. monocytogenes was subjected to low pH stress. Its expression product in E. coli exhibited higher catabolic carbamoyltransfer activities under acidic conditions. Consistently, absence of this enzyme impaired the growth of Listeria under mild acidic condition (pH 4.8) and reduced its survival in synthetic human gastric fluid (pH 2.5), corresponded to a loss in ammonia production, illustrating that Lmo0036 was responsible for acid tolerance at both sublethal and lethal pH levels. Furthermore, Lmo0036 played a possible role in Listeria virulence.
Authors:
Jianshun Chen; Changyong Cheng; Ye Xia; Hanxin Zhao; Chun Fang; Ying Shan; Beibei Wu; Weihuan Fang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-11
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Zhejiang University Institute of Preventive Veterinary Medicine.
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