| Liz1p, a novel fission yeast membrane protein, is required for normal cell division when ribonucleotide reductase is inhibited. | |
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MedLine Citation:
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PMID: 9950674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ribonucleotide reductase activity is required for generating deoxyribonucleotides for DNA replication. Schizosaccharomyces pombe cells lacking ribonucleotide reductase activity arrest during S phase of the cell cycle. In a screen for hydroxyurea-sensitive mutants in S. pombe, we have identified a gene, liz1(+), which when mutated reveals an additional, previously undescribed role for ribonucleotide reductase activity during mitosis. Inactivation of ribonucleotide reductase, by either hydroxyurea or a cdc22-M45 mutation, causes liz1(-) cells in G2 to undergo an aberrant mitosis, resulting in chromosome missegregation and late mitotic arrest. liz1(+) encodes a 514-amino acid protein with strong similarity to a family of transmembrane transporters, and localizes to the plasma membrane of the cell. These results reveal an unexpected G2/M function of ribonucleotide reductase and establish that defects in a transmembrane protein can affect cell cycle progression. |
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Authors:
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E B Moynihan; T Enoch |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular biology of the cell Volume: 10 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-03-18 Completed Date: 1999-03-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 245-57 Citation Subset: IM |
Affiliation:
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Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF052688 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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genetics,
physiology Cell Cycle / genetics, physiology Cell Division / genetics, physiology Cell Membrane / physiology DNA Replication / genetics DNA, Fungal / genetics, metabolism Enzyme Inhibitors / pharmacology Fungal Proteins / genetics, physiology* Gene Deletion Genes, Fungal Hydroxyurea / pharmacology Membrane Proteins / genetics, physiology* Mitosis / drug effects, genetics Mutation Phenotype Ribonucleotide Reductases / antagonists & inhibitors*, genetics Schizosaccharomyces / cytology, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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GM50015/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/DNA, Fungal; 0/Enzyme Inhibitors; 0/Fungal Proteins; 0/Membrane Proteins; 127-07-1/Hydroxyurea; EC 1.17.4.-/Ribonucleotide Reductases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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