| 'Living cantilever arrays' for characterization of mass of single live cells in fluids. | |
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MedLine Citation:
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PMID: 18584076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The size of a cell is a fundamental physiological property and is closely regulated by various environmental and genetic factors. Optical or confocal microscopy can be used to measure the dimensions of adherent cells, and Coulter counter or flow cytometry (forward scattering light intensity) can be used to estimate the volume of single cells in a flow. Although these methods could be used to obtain the mass of single live cells, no method suitable for directly measuring the mass of single adherent cells without detaching them from the surface is currently available. We report the design, fabrication, and testing of 'living cantilever arrays', an approach to measure the mass of single adherent live cells in fluid using silicon cantilever mass sensor. HeLa cells were injected into microfluidic channels with a linear array of functionalized silicon cantilevers and the cells were subsequently captured on the cantilevers with positive dielectrophoresis. The captured cells were then cultured on the cantilevers in a microfluidic environment and the resonant frequencies of the cantilevers were measured. The mass of a single HeLa cell was extracted from the resonance frequency shift of the cantilever and was found to be close to the mass value calculated from the cell density from the literature and the cell volume obtained from confocal microscopy. This approach can provide a new method for mass measurement of a single adherent cell in its physiological condition in a non-invasive manner, as well as optical observations of the same cell. We believe this technology would be very valuable for single cell time-course studies of adherent live cells. |
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Authors:
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Kidong Park; Jaesung Jang; Daniel Irimia; Jennifer Sturgis; James Lee; J Paul Robinson; Mehmet Toner; Rashid Bashir |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-06-11 |
Journal Detail:
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Title: Lab on a chip Volume: 8 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-27 Completed Date: 2008-09-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1034-41 Citation Subset: IM |
Affiliation:
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Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques Cell Size* Cell Survival Hela Cells Humans Microfluidics / methods* Microscopy, Confocal Optics and Photonics Silicon / chemistry* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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P41 EB002503/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-21-3/Silicon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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