| Liver targeting liposomes containing beta-sitosterol glucoside with regard to penetration-enhancing effect on HepG2 cells. | |
| | |
MedLine Citation:
|
PMID: 12081144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of this study was to examine the interaction of soybean-derived sterylglucoside (SG) with the human hepatoblastoma cell line HepG2 with regard to the penetration-enhancing effect of beta-sitosterol glucoside (Sit-G) to clarify the accumulation of SG-containing liposomes (SG-liposomes) to the liver in vivo. The approach was based on measurement of the association of SG-liposomes labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil) in terms of asialoglycoprotein receptor (ASGP-R)-mediated endocytosis, affinity of Sit-G using lAsys and the association of FITC-dextran 4400 (FD-4) increased by Sit-G with the cells. The association of SG-liposomes was decreased by addition of asialofetuin, suggesting that SG-liposomes might be taken up via ASGP-R. Sit-G showed higher affinity with HepG2 cells than HeLa cells, and enhanced the association of FD-4 depending on the incubation time and Sit-G concentrations. Significant positive correlations were found between Sit-G and FD-4 association with the cells, indicating that Sit-G enhanced the drug penetration by distribution in cell membranes. The high degree of liver association of SG-liposomes in vivo might be related to recognition of glucose residues of SG by ASGP-R and to the high affinity and penetration-enhancing effect of Sit-G with hepatocytes. |
| | |
Authors:
|
Kumi Kawano; Kouji Nakamura; Kyoko Hayashi; Tsuneji Nagai; Kozo Takayama; Yoshie Maitani |
Related Documents
:
|
20676434 - Monitoring impedance changes associated with motility and mitosis of a single cell. 2261524 - A new outlook on body fluid viscosity and cell function: concluding remarks and discuss... 2824224 - The improved efficient method for introducing macromolecules into cells using hvj (send... 20213174 - Development of an electrode cell impedance method to measure osteoblast cell activity i... 11458984 - The effect of methyl methanesulfonate (mms)-induced excision repair on p53-dependent ap... 3295064 - A comparative study of spitz nevus and nodular malignant melanoma using image analysis ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological & pharmaceutical bulletin Volume: 25 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2002 Jun |
Date Detail:
|
Created Date: 2002-06-25 Completed Date: 2002-12-31 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
|
Languages: eng Pagination: 766-70 Citation Subset: IM |
Affiliation:
|
Institute of Medicinal Chemistry, Hoshi University, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Asialoglycoprotein Receptor
/
metabolism Asialoglycoproteins / metabolism Binding, Competitive / drug effects Carcinoma, Hepatocellular / metabolism Cell Membrane / drug effects, metabolism Cell Membrane Permeability / drug effects Excipients / metabolism Fluorescein-5-isothiocyanate Humans Liposomes / metabolism* Liver / metabolism* Liver Neoplasms / metabolism Sitosterols / metabolism* Tumor Cells, Cultured alpha-Fetoproteins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Excipients; 0/Liposomes; 0/Sitosterols; 0/alpha-Fetoproteins; 0/asialofetuin; 3326-32-7/Fluorescein-5-isothiocyanate; 474-58-8/lyoniside |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Study on glutathionesulfonic acid sodium salt as biodistribution promoter for thiopental sodium.
Next Document: Cytotoxic effects of 27 anticancer drugs in HeLa and MDR1-overexpressing derivative cell lines.