Document Detail

Liver ribonucleases from the bullfrog, Rana catesbeiana. Purification, properties and changes in activity during metamorphosis.
MedLine Citation:
PMID:  313217     Owner:  NLM     Status:  MEDLINE    
A major and three minor RNAases exhibiting activity at neutral pH were found in bullfrog liver homogenates. These RNAases were purified to electrophoretic homogeneity. Total purification was 1100 to 1600-fold, and the average recovery was 35%. The molecular weight estimated by gel filtration and SDS-urea-polyacrylamide gel electrophoresis was 12,000 for all the components. An antibody against the major RNAase (component B) reacted with the major RNAase to form a single precipitin line on double immunodiffusion and inhibited the activity of the major RNAase but did not cross-react with the other three components and bovine pancreatic RNAase A. Amino acid analyses revealed that the major RNAase differed markedly from the other three RNAase components in the contents of ten amino acid residues, whereas the minor RNAases were similar to each other. The major RNAase was contained in both frog and tadpole liver and its activity in the liver markedly increased at the metamorphic climax.
Y Tomita; Y Goto; T Okazaki; R Shukuya
Related Documents :
1126917 - Characterization of an inhibitor of ribonucleic acid polymerase from the mycelial phase...
19364487 - Rapid sequencing gel electrophoresis using glycerol-tolerant sodium taurine medium.
16658117 - Purification and partial characterization of a glucan containing indole-3-acetic acid.
2903587 - Isolation and characterization of three lethal and hemolytic toxins from the sea anemon...
6184857 - Sonication and other improvements on jeffrey's technique for macerating wood.
22062767 - Effect of days on concentrate feed on sensory off-flavor score, off-flavor descriptor a...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  562     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  504-14     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Chromatography / methods
Isoelectric Point
Liver / enzymology*
Metamorphosis, Biological*
Molecular Weight
Rana catesbeiana / physiology*
Ribonucleases / analysis,  isolation & purification,  metabolism*
Reg. No./Substance:
0/Amino Acids; EC 3.1.-/Ribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Histiocytosis X in two infants--treated with topical nitrogen mustard.
Next Document:  Studies on factor VIII-related protein. I. Ultrastructural and electrophoretic heterogeneity of huma...