Document Detail

Liver growth factor treatment restores cell-extracellular matrix balance in resistance arteries and improves left ventricular hypertrophy in SHR.
MedLine Citation:
PMID:  21642499     Owner:  NLM     Status:  Publisher    
Liver growth factor (LGF) is an endogenous albumin-bilirubin complex with antihypertensive effects in spontaneously hypertensive rats (SHR). We assessed the actions of LGF treatment on SHR mesenteric resistance and intramyocardial arteries (MRA,IMA), heart and Vascular Smooth Muscle Cells (VSMC). SHR and Wistar Kyoto (WKY) rats, treated with vehicle or LGF (4.5 μg LGF/rat, 4 i.p. injections over 12 days) were used. Intra-arterial blood pressure was measured in anaesthetized rats. Heart was weighted and paraffin-embedded. Proliferation, ploidy and fibronectin deposition were studied in carotid artery-derived VSMC by immuno-cytochemistry. In MRA we assessed: 1) geometry and mechanics by pressure myography; 2) function by wire myography; 3) collagen by sirius red-staining and polarized light microscopy and 4) elastin, cell density, nitric oxide (NO) and superoxide anion by confocal microscopy. Heart sections were used to assess cell density and collagen content in IMA. Left ventricular hypertrophy (LVH) regression was assessed by echocardiography. LGF reduced blood pressure only in SHR. LGF -in vitro or as treatment- normalized the alterations in proliferation and fibronectin in SHR-derived VSMC with no effect on WKY cells. In MRA, LGF treatment normalized collagen, elastin and VSMC content and passive mechanical properties. In addition, it improved NO availability through reduction of superoxide anion. In IMA LGF treatment normalized perivascular collagen and VSMC density improving wall:lumen ratio. Paired experiments demonstrated a partial regression of SHR LVH by LGF treatment. The effective cardiovascular antifibrotic and regenerative actions of LGF support its potential in the treatment of hypertension and its complications.
M Victoria Conde; M Carmen Gonzalez; Begoña Quintana-Villamandos; Fatima Abderrahim; Ana Maria Briones; Luis A Condezo-Hoyos; Javier Regadera; Cristina Susin; Jose J Gomez de Diego; Emilio Delgado-Baeza; Juan J Díaz-Gil; Silvia M Arribas
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-3
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  -     ISSN:  1522-1539     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Universidad Autonoma de Madrid.
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