Document Detail


Liver denervation affects hepatocyte mitochondrial fatty acid transport capacity.
MedLine Citation:
PMID:  14695648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of liver denervation on the activity of hepatic carnitine palmitoyltransferase (CPT) system, which catalyses the transfer of long-chain fatty acids into the mitochondria, was studied in rats. Noradrenaline content in phenol-denervated liver (D) was reduced by 87%. CPT I and II activities (measured by radioassay after detergent separation of the enzymes) were decreased (p < 0.001) in D (2.6 +/- 0.1 and 0.68 +/- 0.2 nmol min(-1) mg(-1) protein, respectively) as compared with controls (4.7 +/- 0.3 and 2.5 +/- 0.2 nmol min(-1) mg(-1) protein, for CPT I and II, respectively). A less intense immunoreactive band for denervated liver CPT II was obtained after Western blotting. Concomitantly, long-chain fatty acid incorporation (p < 0.001), evaluated after administration of [14C]-oleate and total fat content (p < 0.001) were increased in D in relation to controls, while incorporation of exogenous [14C]-oleate into secreted VLDL, was decreased (p < 0.01). The effect of sympathetic denervation on CPT activity was different from that evoked by adrenodemedullation, which caused an augmentation of CPT activity (p < 0.01), when compared with the liver of intact rats. The effects of denervation and adrenodemedullation on the other parameters of lipid metabolism studied, were similar. The results strongly suggest a role of liver sympathetic innervation in the regulation of liver lipid metabolism.
Authors:
Flávia R Carreño; Marília C L Seelaender
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  22     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2003-12-25     Completed Date:  2004-08-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  9-17     Citation Subset:  IM    
Copyright Information:
Copyright 2003 John Wiley & Sons, Ltd.
Affiliation:
Laboratory of Histophysiology, Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Medulla / metabolism
Animals
Biological Transport / physiology
Blotting, Western
Carnitine O-Palmitoyltransferase / metabolism
Denervation*
Fatty Acids / metabolism*
Hepatocytes / metabolism*
Lipid Metabolism
Liver / innervation*
Mitochondria, Liver / metabolism*
Oleic Acid / metabolism
Rats
Rats, Wistar
Triolein / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 112-80-1/Oleic Acid; 122-32-7/Triolein; EC 2.3.1.21/Carnitine O-Palmitoyltransferase

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