| Liver cell damage and lysosomal iron storage in patients with idiopathic hemochromatosis. A light and electron microscopic study. | |
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MedLine Citation:
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PMID: 2254628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eleven patients with idiopathic hemochromatosis were subjected to percutaneous liver biopsy, and seven were rebiopsied after repeated phlebotomies. The liver tissue was examined by light and electron microscopy. Ultrastructural morphometry was also performed and iron content was determined. Initially, all biopsies displayed large iron-laden lysosomes in the hepatocytes. Lysosomal volume density was increased and correlated well with liver iron content (r = 0.84, p less than 0.005). Neither mitochondria nor the endoplasmic reticulum showed any ultrastructural changes, except in the necrotic cells of biopsies with the highest liver iron content. In these cases, iron-laden lysosomes were also encountered in the Kupffer cells. Following treatment, liver iron content and lysosomal volume density were normalized. More specifically, iron content was 14.1 +/- 2.1 micrograms Fe/mg protein before and 1.3 +/- 0.3 micrograms Fe/mg protein after treatment (p less than 0.001). Lysosomal volume density was 6.1 +/- 0.8% before and 1.8 +/- 0.2% after treatment (p less than 0.001). Hence, in the precirrhotic stage of idiopathic hemochromatosis, the first evident ultrastructural changes are in the lysosomal compartment. These changes correlate well with the iron overload, also in advanced stages of the disease, and are reversed after iron removal. |
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Authors:
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P Stål; H Glaumann; R Hultcrantz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of hepatology Volume: 11 ISSN: 0168-8278 ISO Abbreviation: J. Hepatol. Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1991-01-24 Completed Date: 1991-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 172-80 Citation Subset: IM |
Affiliation:
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Department of Medicine, Karolinska Institute, Huddinge Hospital, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Female Hemochromatosis / etiology*, metabolism, pathology Humans Iron / metabolism* Liver / metabolism, pathology*, ultrastructure Lysosomes / metabolism*, ultrastructure Male Microscopy, Electron Middle Aged Organelles / metabolism, ultrastructure |
| Chemical | |
Reg. No./Substance:
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7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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