| Liver and adipose tissue contributions to newly formed fatty acids in an ascites tumor. | |
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MedLine Citation:
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PMID: 6742224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We determined the contribution from host hepatic and extrahepatic tissues to newly synthesized fatty acids (FA) in the Ehrlich ascites tumor (EAT). We administered 3H2O (subcutaneously) and [14C]glucose (in a test meal) and measured the appearance of radioactivity in plasma triglyceride fatty acids (TGFA) and free fatty acids (FFA) and in tumor total lipid fatty acids (TLFA). Using [14 C]FFA, we selectively labeled epididymal fat TGFA to estimate the FA transport rate from intraperitoneal adipose tissue directly to the tumor. Contributions of four major pathways to newly synthesized FA in EAT were determined by multicompartmental analysis. De novo FA synthesis by EAT accounted for more than 93% of the TLFA radioactivity found in the tumor. Contributions from liver TGFA via plasma TGFA (less than 0.5%), adipose tissue TGFA via plasma FFA (less than 6%), and adipose tissue TGFA via direct intraperitoneal transport of FFA (less than 1%) accounted for less than 7% of all TLFA radioactivity measured in the EAT. Thus the present study establishes that practically all labeled esterified FA in the EAT is derived from de novo synthesis by tumor cells. |
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Authors:
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M Ookhtens; R Kannan; I Lyon; N Baker |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 247 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-08-17 Completed Date: 1984-08-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R146-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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metabolism* Animals Biological Transport Carbon Radioisotopes / diagnostic use Carcinoma, Ehrlich Tumor / metabolism* Fatty Acids / biosynthesis, metabolism* Fatty Acids, Nonesterified / metabolism Glucose / diagnostic use Liver / metabolism* Male Mice Models, Biological Scintillation Counting Triglycerides / metabolism Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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CA-15813/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Triglycerides; 10028-17-8/Tritium; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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