Document Detail


Liver and adipose tissue contributions to newly formed fatty acids in an ascites tumor.
MedLine Citation:
PMID:  6742224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We determined the contribution from host hepatic and extrahepatic tissues to newly synthesized fatty acids (FA) in the Ehrlich ascites tumor (EAT). We administered 3H2O (subcutaneously) and [14C]glucose (in a test meal) and measured the appearance of radioactivity in plasma triglyceride fatty acids (TGFA) and free fatty acids (FFA) and in tumor total lipid fatty acids (TLFA). Using [14 C]FFA, we selectively labeled epididymal fat TGFA to estimate the FA transport rate from intraperitoneal adipose tissue directly to the tumor. Contributions of four major pathways to newly synthesized FA in EAT were determined by multicompartmental analysis. De novo FA synthesis by EAT accounted for more than 93% of the TLFA radioactivity found in the tumor. Contributions from liver TGFA via plasma TGFA (less than 0.5%), adipose tissue TGFA via plasma FFA (less than 6%), and adipose tissue TGFA via direct intraperitoneal transport of FFA (less than 1%) accounted for less than 7% of all TLFA radioactivity measured in the EAT. Thus the present study establishes that practically all labeled esterified FA in the EAT is derived from de novo synthesis by tumor cells.
Authors:
M Ookhtens; R Kannan; I Lyon; N Baker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  247     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-08-17     Completed Date:  1984-08-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R146-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism*
Animals
Biological Transport
Carbon Radioisotopes / diagnostic use
Carcinoma, Ehrlich Tumor / metabolism*
Fatty Acids / biosynthesis,  metabolism*
Fatty Acids, Nonesterified / metabolism
Glucose / diagnostic use
Liver / metabolism*
Male
Mice
Models, Biological
Scintillation Counting
Triglycerides / metabolism
Tritium / diagnostic use
Grant Support
ID/Acronym/Agency:
CA-15813/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Triglycerides; 10028-17-8/Tritium; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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