Document Detail

Liver X receptor alpha mediated genistein induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1) in Hep G2 cells.
MedLine Citation:
PMID:  23352501     Owner:  NLM     Status:  MEDLINE    
Cytosolic sulfotransferases are one of the major families of phase II drug metabolizing enzymes. Sulfotransferase-catalyzed sulfonation regulates hormone activities, metabolizes drugs, detoxifies xenobiotics, and bioactivates carcinogens. Human dehydroepiandrosterone sulfotransferase (hSULT2A1) plays important biological roles by sulfating endogenous hydroxysteroids and exogenous xenobiotics. Genistein, mainly existing in soy food products, is a naturally occurring phytoestrogen with both chemopreventive and chemotherapeutic potential. Our previous studies have shown that genistein significantly induces hSULT2A1 in Hep G2 and Caco-2 cells. In this study, we investigated the roles of liver X receptor (LXRα) in the genistein induction of hSULT2A1. LXRs have been shown to induce expression of mouse Sult2a9 and hSULT2A1 gene. Our results demonstrate that LXRα mediates the genistein induction of hSULT2A1, supported by Western blot analysis results, hSULT2A1 promoter driven luciferase reporter gene assay results, and mRNA interference results. Chromatin immunoprecipitation (ChIP) assay results demonstrate that genistein increase the recruitment of hLXRα binding to the hSULT2A1 promoter. These results suggest that hLXRα plays an important role in the hSULT2A1 gene regulation. The biological functions of phytoestrogens may partially relate to their induction activity toward hydroxysteroid SULT.
Yue Chen; Shunfen Zhang; Tianyan Zhou; Chaoqun Huang; Alicia McLaughlin; Guangping Chen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-23
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  268     ISSN:  1096-0333     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-05-10     Revised Date:  2014-04-16    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-12     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
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MeSH Terms
Chromatin Immunoprecipitation
Enzyme Induction / drug effects
Genistein / pharmacology*
Hep G2 Cells
Hydrocarbons, Fluorinated / pharmacology
Orphan Nuclear Receptors / physiology*
Promoter Regions, Genetic
Sulfonamides / pharmacology
Sulfotransferases / biosynthesis*,  genetics
Grant Support
Reg. No./Substance:
0/Hydrocarbons, Fluorinated; 0/Orphan Nuclear Receptors; 0/Sulfonamides; 0/TO-901317; 0/liver X receptor; DH2M523P0H/Genistein; EC 2.8.2.-/Sulfotransferases; EC sulfotransferase

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