Document Detail

Liver L-carnitine biosynthesis in non-pregnant, pregnant and lactating rabbits.
MedLine Citation:
PMID:  15244037     Owner:  NLM     Status:  MEDLINE    
The present study reports liver carnitine biosynthesis and concentrations in non-pregnant (dry), pregnant and lactating rabbits (n = 17). To determine carnitine biosynthesis in liver tissues; we purified gamma-butyrobetaine hydroxylase, a key enzyme in carnitine biosynthesis, and measured its activity. An isocratic high-performance liquid chromatography (HPLC) method was used to determine L-carnitine levels in liver samples. When compared with dry period, during pregnancy, free and acid-soluble total carnitine concentrations did not change significantly (p>0.05). In contrast to free and acid-soluble total carnitine, short chain acylcarnitine concentration increased significantly in the second period of pregnancy, compared with non-pregnant rabbits. Highest concentrations of free, short-chain acyl- and acid-soluble total carnitine were measured at the 4th day of lactation (487 +/- 29; 392 +/- 26 and 879 +/- 38 nmol/g wet weight respectively). gamma-Butyrobetaine hydroxylase activity increased with the duration of pregnancy and in late pregnancy it increased significantly compared to previous periods. Highest activity was measured in early lactation period (361 +/- 28 pmol carnitine/min/mg protein). We concluded that, biosynthesis of carnitine in liver increases gradually in late pregnancy and early lactation in rabbit, and this may be regulated by hormonal changes during these periods.
Abdurrahman Coşkun; Mahmut Doğan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research communications in molecular pathology and pharmacology     Volume:  111     ISSN:  1078-0297     ISO Abbreviation:  Res. Commun. Mol. Pathol. Pharmacol.     Publication Date:  2002  
Date Detail:
Created Date:  2004-07-12     Completed Date:  2004-08-09     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9437512     Medline TA:  Res Commun Mol Pathol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  211-23     Citation Subset:  IM    
Kirikkale State Hospital, Department of Clinical Biochemistry, Kirikkale, Turkey.
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MeSH Terms
Carnitine / biosynthesis*
Chromatography, High Pressure Liquid
Lactation / metabolism*
Liver / enzymology,  metabolism*
Mixed Function Oxygenases / isolation & purification,  metabolism
Pregnancy, Animal / metabolism*
Spectrophotometry, Ultraviolet
gamma-Butyrobetaine Dioxygenase
Reg. No./Substance:
541-15-1/Carnitine; EC 1.-/Mixed Function Oxygenases; EC Dioxygenase

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