Document Detail

Live single-cell molecular analysis by video-mass spectrometry.
MedLine Citation:
PMID:  18469458     Owner:  NLM     Status:  MEDLINE    
The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.
Naohiro Tsuyama; Hajime Mizuno; Emi Tokunaga; Tsutomu Masujima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  24     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-08-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  559-61     Citation Subset:  IM    
Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
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MeSH Terms
Cell Survival
Mass Spectrometry / methods*
Molecular Structure
Proline / chemistry
Swiss 3T3 Cells
Videotape Recording / methods*
Reg. No./Substance:

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