Document Detail


Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation.
MedLine Citation:
PMID:  22529358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcription of genes can be discontinuous, occurring in pulses or bursts. It is not clear how properties of transcriptional pulses vary between different genes. We compared the pulsing of five housekeeping and five developmentally induced genes by direct imaging of single gene transcriptional events in individual living Dictyostelium cells. Each gene displayed its own transcriptional signature, differing in probability of firing and pulse duration, frequency, and intensity. In contrast to the prevailing view from both prokaryotes and eukaryotes that transcription displays binary behavior, strongly expressed housekeeping genes altered the magnitude of their transcriptional pulses during development. These nonbinary "tunable" responses may be better suited than stochastic switch behavior for housekeeping functions. Analysis of RNA synthesis kinetics using fluorescence recovery after photobleaching implied modulation of housekeeping-gene pulse strength occurs at the level of transcription initiation rather than elongation. In addition, disparities between single cell and population measures of transcript production suggested differences in RNA stability between gene classes. Analysis of stability using RNAseq revealed no major global differences in stability between developmental and housekeeping transcripts, although strongly induced RNAs showed unusually rapid decay, indicating tight regulation of expression.
Authors:
Tetsuya Muramoto; Danielle Cannon; Marek Gierlinski; Adam Corrigan; Geoffrey J Barton; Jonathan R Chubb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-23
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-09     Completed Date:  2012-08-13     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7350-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Blotting, Northern
Dictyostelium / cytology,  genetics*,  growth & development
Fluorescence Recovery After Photobleaching
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Genes, Protozoan / genetics
Kinetics
Models, Genetic
RNA Stability
RNA, Protozoan / genetics*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Single-Cell Analysis / methods
Transcription, Genetic / genetics*
Grant Support
ID/Acronym/Agency:
090904//Wellcome Trust; G0600242//Medical Research Council; G120/1013//Medical Research Council; G120/1013(75407)//Medical Research Council; //Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/RNA, Protozoan
Comments/Corrections

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