Document Detail

Live imaging of dendritic cell-Treg cell interactions.
MedLine Citation:
PMID:  21287331     Owner:  NLM     Status:  MEDLINE    
The decision to launch an immune response is made during the interaction of helper T cells and regulatory T cells with dendritic cells. Recognition of antigen leads to formation of immunological synapses at the interface between the cells and to activation of the T cells. The length of interaction between the T cells and dendritic cells influences the functional outcome. We have shown that in the absence of proinflammatory stimuli, regulatory T cells and naive helper T cells interact differently with dendritic cells. Neuropilin-1, which is expressed by most regulatory T cells but not naive helper T cells, promotes prolonged interactions with immature dendritic cells, resulting in higher sensitivity to limiting amounts of antigen. We tracked T cell-dendritic cell interactions in real-time using time-lapse microscopy, assessed synapse formation by immunofluorescence, and measured regulatory T cell activation by dendritic cells using suppression assays.
Milka Sarris; Alexander G Betz
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  707     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-02-02     Completed Date:  2011-05-02     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-101     Citation Subset:  IM    
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MeSH Terms
Dendritic Cells / cytology*,  metabolism*
Microscopy, Confocal / methods*
T-Lymphocytes, Regulatory / cytology*,  metabolism*
Grant Support
MC_U105184296//Medical Research Council

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