| Live imaging of cell extrusion from the epidermis of developing zebrafish. | |
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MedLine Citation:
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PMID: 21730948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homeostatic maintenance of epithelial tissues requires the continual removal of damaged cells without disrupting barrier function. Our studies have found that dying cells send signals to their live neighbors to form and contract a ring of actin and myosin that ejects it out from the epithelial sheet while closing any gaps that might have resulted from its exit, a process termed cell extrusion¹. The optical clarity of developing zebrafish provides an excellent system to visualize extrusion in living epithelia. Here we describe a method to induce and image extrusion in the larval zebrafish epidermis. To visualize extrusion, we inject a red fluorescent protein labeled probe for F-actin into one-cell stage transgenic zebrafish embryos expressing green fluorescent protein in the epidermis and induce apoptosis by addition of G418 to larvae. We then use time-lapse imaging on a spinning disc confocal microscope to observe actin dynamics and epithelial cell behaviors during the process of apoptotic cell extrusion. This approach allows us to investigate the extrusion process in live epithelia and will provide an avenue to study disease states caused by the failure to eliminate apoptotic cells. |
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Authors:
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George T Eisenhoffer; Jody Rosenblatt |
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Publication Detail:
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Type: Research Support, N.I.H., Extramural; Video-Audio Media Date: 2011-06-27 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2011 |
Date Detail:
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Created Date: 2011-07-06 Completed Date: 2011-07-25 Revised Date: 2011-10-26 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis,
metabolism Animals Animals, Genetically Modified Apoptosis / drug effects, physiology* Epidermis / cytology*, drug effects, metabolism Gentamicins / pharmacology Green Fluorescent Proteins / analysis, biosynthesis, genetics Luminescent Proteins / analysis Microscopy, Confocal / methods Zebrafish / embryology* |
| Grant Support | |
ID/Acronym/Agency:
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1 DP2OD002056-01/OD/NIH HHS; 5T32 CA03247-8/CA/NCI NIH HHS; DP2 OD002056-01/OD/NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Gentamicins; 0/Luminescent Proteins; 0/red fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 49863-47-0/antibiotic G 418 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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