| Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure. | |
| | |
MedLine Citation:
|
PMID: 22162136 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Many cells die during development, tissue homeostasis, and disease. Dysregulation of apoptosis leads to cranial neural tube closure (NTC) defects like exencephaly, although the mechanism is unclear. Observing cells undergoing apoptosis in a living context could help elucidate their origin, behavior, and influence on surrounding tissues, but few tools are available for this purpose, especially in mammals. In this paper, we used insulator sequences to generate a transgenic mouse that stably expressed a genetically encoded fluorescence resonance energy transfer (FRET)-based fluorescent reporter for caspase activation and performed simultaneous time-lapse imaging of apoptosis and morphogenesis in living embryos. Live FRET imaging with a fast-scanning confocal microscope revealed that cells containing activated caspases showed typical and nontypical apoptotic behavior in a region-specific manner during NTC. Inhibiting caspase activation perturbed and delayed the smooth progression of cranial NTC, which might increase the risk of exencephaly. Our results suggest that caspase-mediated cell removal facilitates NTC completion within a limited developmental window. |
| | |
Authors:
|
Yoshifumi Yamaguchi; Naomi Shinotsuka; Keiko Nonomura; Kiwamu Takemoto; Keisuke Kuida; Hiroki Yosida; Masayuki Miura |
Related Documents
:
|
7915976 - Primary t lymphocyte immunodeficiency associated with a selective impairment of cd2, cd... 9743616 - Regulation of human lymphocyte il-4 secretion by intestinal epithelial cell-derived int... 9568616 - In vivo expression of cytokine mrna in rats infected with schistosoma mansoni. 12372936 - B7-1 (cd80) and b7-2 (cd 86) expression in human tubular epithelial cells in vivo and i... 16133866 - Synergistic apoptosis induction by proteasome and histone deacetylase inhibitors is dep... 16317506 - Characterization of cell clones stably transfected with short form caspase-9: apoptotic... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of cell biology Volume: 195 ISSN: 1540-8140 ISO Abbreviation: J. Cell Biol. Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 1047-60 Citation Subset: IM |
Affiliation:
|
Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Ne...
Next Document: A novel PET tracer for the imaging of ?v?3 and ?v?5 integrins in experimental breast cancer bone met...