Document Detail


Live imaging of the Dictyostelium cell cycle reveals widespread S phase during development, a G2 bias in spore differentiation and a premitotic checkpoint.
MedLine Citation:
PMID:  18367554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regulation of the Dictyostelium cell cycle has remained ambiguous owing to difficulties in long-term imaging of motile cells and a lack of markers for defining cell cycle phases. There is controversy over whether cells replicate their DNA during development, and whether spores are in G1 or G2 of the cell cycle. We have introduced a live-cell S-phase marker into Dictyostelium cells that allows us to precisely define cycle phase. We show that during multicellular development, a large proportion of cells undergo nuclear DNA synthesis. Germinating spores enter S phase only after their first mitosis, indicating that spores are in G2. In addition, we demonstrate that Dictyostelium heterochromatin is copied late in S phase and replicates via accumulation of replication factors, rather than recruitment of DNA to pre-existing factories. Analysis of variability in cycle times indicates that regulation of the cycle manifests at a single random transition in G2, and we present the first identified checkpoint in Dictyostelium, which operates at the G2-M transition in response to DNA damage.
Authors:
Tetsuya Muramoto; Jonathan R Chubb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-26
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  135     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-08-15     Revised Date:  2013-04-29    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1647-57     Citation Subset:  IM    
Affiliation:
Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cell Differentiation / physiology*
Cell Proliferation
DNA Damage
DNA Replication / physiology
DNA, Protozoan / biosynthesis
Dictyostelium / cytology,  growth & development,  physiology*
Fluorometry
G2 Phase / physiology
Heterochromatin / physiology
Mitosis / physiology
Proliferating Cell Nuclear Antigen / metabolism
S Phase / physiology
Spores, Protozoan / cytology,  growth & development,  physiology
Grant Support
ID/Acronym/Agency:
G120/1013//Medical Research Council; G120/1013(75407)//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/DNA, Protozoan; 0/Heterochromatin; 0/Proliferating Cell Nuclear Antigen

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