| Live imaging of the Dictyostelium cell cycle reveals widespread S phase during development, a G2 bias in spore differentiation and a premitotic checkpoint. | |
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MedLine Citation:
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PMID: 18367554 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The regulation of the Dictyostelium cell cycle has remained ambiguous owing to difficulties in long-term imaging of motile cells and a lack of markers for defining cell cycle phases. There is controversy over whether cells replicate their DNA during development, and whether spores are in G1 or G2 of the cell cycle. We have introduced a live-cell S-phase marker into Dictyostelium cells that allows us to precisely define cycle phase. We show that during multicellular development, a large proportion of cells undergo nuclear DNA synthesis. Germinating spores enter S phase only after their first mitosis, indicating that spores are in G2. In addition, we demonstrate that Dictyostelium heterochromatin is copied late in S phase and replicates via accumulation of replication factors, rather than recruitment of DNA to pre-existing factories. Analysis of variability in cycle times indicates that regulation of the cycle manifests at a single random transition in G2, and we present the first identified checkpoint in Dictyostelium, which operates at the G2-M transition in response to DNA damage. |
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Authors:
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Tetsuya Muramoto; Jonathan R Chubb |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-26 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 135 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-14 Completed Date: 2008-08-15 Revised Date: 2013-04-29 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 1647-57 Citation Subset: IM |
Affiliation:
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Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / physiology* Cell Differentiation / physiology* Cell Proliferation DNA Damage DNA Replication / physiology DNA, Protozoan / biosynthesis Dictyostelium / cytology, growth & development, physiology* Fluorometry G2 Phase / physiology Heterochromatin / physiology Mitosis / physiology Proliferating Cell Nuclear Antigen / metabolism S Phase / physiology Spores, Protozoan / cytology, growth & development, physiology |
| Grant Support | |
ID/Acronym/Agency:
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G120/1013//Medical Research Council; G120/1013(75407)//Medical Research Council; //Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan; 0/Heterochromatin; 0/Proliferating Cell Nuclear Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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