Document Detail

Live fluorescence and transmission-through-dye microscopic study of actinomycin D-induced apoptosis and apoptotic volume decrease.
MedLine Citation:
PMID:  23325449     Owner:  NLM     Status:  Publisher    
The effect of actinomycin D on HeLa cells was studied by live fluorescence and transmission-through-dye microscopy-a recently developed technique that permits volume measurements in live cells. In particular, it is well suited for the observation and quantification of the apoptotic volume decrease (AVD), which is widely viewed as an essential feature of apoptosis. The main results from our study are as follows. (1) Apoptosis caused in HeLa cells by actinomycin D proceeds in two morphologically distinct stages: the early stage is characterized by extensive blebbing, and the late stage by a more compact shape. The loss of mitochondrial membrane potential occurs at about the same time as blebbing, and chromatin condensation follows 30-90 min later. Caspase-3 and 7 become activated during the late stage. (2) Because blebbing occurs before activation of caspase-3, it has to be initiated by a different mechanism. Although blebbing is one of the earliest observable changes, it can be selectively inhibited without affecting other apoptotic reactions. (3) The majority of cells experience a temporary volume increase after the appearance of blebs. Eventually, AVD takes over and the cells shrink by approximately 40 % of their initial volume; the volume loss becomes noticeable at the end of the blebbing phase and continues through the late stage. Sometimes, at the end of long incubations, shrinkage gives way to swelling, possibly indicating secondary necrosis. (4) Both early and late apoptosis are accompanied by intracellular accumulation of Na(+), while low-sodium medium prevents apoptosis. Except for a partial protective effect of quinine, all of the tested blockers of Na(+), K(+) and Cl(-) channels failed to prevent apoptosis or AVD.
Neda R Kasim; Kateřina Kuželová; Aleš Holoubek; Michael A Model
Related Documents :
23606539 - The novel role of il-7 ligation to il-7 receptor in myeloid cells of rheumatoid arthrit...
24102579 - Ebola virus-like particles stimulate type i interferons and proinflammatory cytokine ex...
23090609 - Nitric oxide synthase 2 is required for conversion of pro-fibrogenic inflammatory cd133...
4044099 - Depression of con a proliferative response of immune cells by in vitro hyperoxic exposu...
25071759 - Embryonic stem cells promoting macrophage survival and function are crucial for teratom...
23317509 - Revisiting immune-based therapies for aggressive follicular cell-derived thyroid cancers.
18437699 - Ni(ii) ions dysregulate cytokine secretion from human monocytes.
20443159 - Fighting cancer with red wine? molecular mechanisms of resveratrol.
25007519 - Expression of xbp1 in lymphocytes of small intestine rats under chronic social stress a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  -     ISSN:  1573-675X     ISO Abbreviation:  Apoptosis     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biological Sciences, Kent State University, Kent, OH, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Astragaloside IV prevents acute kidney injury in two rodent models by inhibiting oxidative stress an...
Next Document:  Icosapent Ethyl, a Pure Ethyl Ester of Eicosapentaenoic Acid: Effects on Circulating Markers of Infl...