| Live birth following intracytoplasmic sperm injection after a 15-hour weather-related delay in cryopreservation of testicular sperm. | |
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MedLine Citation:
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PMID: 15705394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To report a live birth after transfer of cryopreserved blastocysts derived from testicular sperm that were cryopreserved 15 hours after biopsy due to inclement weather. DESIGN: Case report. SETTING: University-affiliated private clinic. PATIENT(S): Couple undergoing IVF with intracytoplasmic sperm injection (ICSI) using testicular extracted sperm for male factor infertility due to unexplained obstructive azoospermia. INTERVENTION(S): An IVF case involving testicular biopsy, cryopreservation, and thawing of testicular tissue, ICSI, embryo freezing, and transfer of cyropreserved blastocysts. MAIN OUTCOME MEASURE(S): The ability to use cryopreserved testicular tissue in which optimal time from biopsy to freezing had been delayed because the weather was inclement. RESULT(S): In spite of a 15-hour delay in processing and cryopreservation of the testicular biopsy, subsequent thawing yielded viable sperm that resulted in viable embryos after ICSI. Transfer of fresh embryos was unsuccessful. However, subsequent use of supernumerary viable blastocysts that were frozen resulted in the live birth of a baby. CONCLUSION(S): A 15-hour delay in processing and cryopreservation of a testicular biopsy did not affect the viability of the extracted sperm after thawing. Although it is desirable to freeze the testicular specimen as soon as possible, the delay in processing in this case due to inclement weather did not affect the potential for fertilization and implantation of a viable cryopreserved blastocyst. |
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Authors:
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Hemlata S Bhakoo; Peter Vaccaro; Kent Crickard; Michael W Sullivan |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: Fertility and sterility Volume: 83 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-11 Completed Date: 2005-04-05 Revised Date: 2005-12-27 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 464-5 Citation Subset: IM |
Affiliation:
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Infertility and IVF Medical Associates of WNY, 4510 Main Street, Snyder, NY 14226, USA. hsbhakoo@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biopsy Blastomeres Cryopreservation* Female Humans Infant, Newborn Male Pregnancy Pregnancy Outcome* Semen Preservation* Sperm Injections, Intracytoplasmic* Spermatozoa / cytology* Testis / cytology Time Factors Weather |
| Comments/Corrections | |
Comment In:
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Fertil Steril. 2005 Aug;84(2):547-8; author reply 548
[PMID:
16084914
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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