| Live colonocytes in newborn stool: surrogates for evaluation of gut physiology and disease pathogenesis. | |
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MedLine Citation:
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PMID: 21544008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies of gastrointestinal pathophysiology are not feasible by biopsies in human neonates. We examined the utility of live colonocytes in stool in studying cellular markers during early neonatal life. Expression of IgA, IgG, cluster of differentiation-45 cells (CD45), and toll-like receptors-2 and 4 (TLR2 and TLR4) were analyzed by flow cytometry. Colonocyte RNA extracts were used in quantitative real-time PCR (qRT-PCR) to examine the expression of cytokeratin-19, ribosomal protein-24, and tight-junction (Tj) protein zonula occludens-1 (ZO-1). Colonocyte yield varied between 5 × 10⁴ to 2 × 10⁶ cells/g of stool. Meconium samples yielded a highly enriched population of viable cells. Although low, all samples showed CD45-positive cells during the initial weeks of life. Starting as early as d 2, IgA expression was observed in 69% of the cells. Low to moderate expression of IgG was observed with a linear increase as the infants grew. There was an almost total lack of TLR2 staining; however, >55% of the colonocytes showed TLR4 expression. Although high levels of IgA in gut cells may serve as a natural protectant during neonatal period, increased TLR4 may provide a niche for lipopolysaccharide (LPS)-mediated epithelial damage. Use of stool colonocytes can be a valuable noninvasive approach for studying gut pathophysiology in the neonatal period. |
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Authors:
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Dinesh S Chandel; Gheorghe T Braileanu; June-Home J Chen; Hegang H Chen; Pinaki Panigrahi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Pediatric research Volume: 70 ISSN: 1530-0447 ISO Abbreviation: Pediatr. Res. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-07 Completed Date: 2011-11-15 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 153-8 Citation Subset: IM |
Affiliation:
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Department of Environmental, Agricultural and Occupational Health, Center for Global Health and Development, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD45
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metabolism Biological Markers / metabolism* Colon / cytology* Epithelial Cells / cytology, metabolism* Feces / cytology* Flow Cytometry Gastrointestinal Tract / metabolism, physiology*, physiopathology* Humans Immunoglobulin A / metabolism Immunoglobulin G / metabolism Infant, Newborn Keratin-19 / metabolism Membrane Proteins / metabolism Phosphoproteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Ribosomal Proteins / metabolism Toll-Like Receptor 2 / metabolism Toll-Like Receptor 4 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD053719-01A1/HD/NICHD NIH HHS; R01HD53719/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Keratin-19; 0/Membrane Proteins; 0/Phosphoproteins; 0/Ribosomal Proteins; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/zonula occludens-1 protein; EC 3.1.3.48/Antigens, CD45 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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