Document Detail

Lithospermic acid B is more responsive to silver ions (Ag+) than rosmarinic acid in Salvia miltiorrhiza hairy root cultures.
MedLine Citation:
PMID:  19210264     Owner:  NLM     Status:  MEDLINE    
LAB (lithospermic acid B) is a dimer of RA (rosmarinic acid) and has been suggested to be derived from RA, but the detailed biosynthesis process has not yet been identified. The accumulation of RA has been intensively investigated in the plant species of Boraginaceae and Lamiaceae. In the present study, we report that silver ions (Ag+; 15 microM), an abiotic elicitor, did not stimulate RA accumulation but dramatically enhanced LAB from approx. 5.4% to 18.8% of dry weight in Salvia miltiorrhiza hairy root cultures, and the rise in LAB was found to be coincident with the decline of RA content at each time point after treatment. Meanwhile, a profiling analysis of genes and metabolites (intermediates) involved in the RA synthesis pathway was performed; the result indicated that several gene transcripts and metabolite accumulations show temporal changes in abundance consistent with LAB production. Thus a potential (putative) biosynthetic route from RA to LAB was presumed, which was suggested to be significantly activated by Ag+ in S. miltiorrhiza hairy root cultures. Further intermediate monitoring and compound feeding experiments were performed to rank the strength of this hypothesis. Our study, for the first time, provides evidence that RA is a precursor leading to LAB synthesis.
Ying Xiao; Shouhong Gao; Peng Di; Junfeng Chen; Wansheng Chen; Lei Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-06
Journal Detail:
Title:  Bioscience reports     Volume:  30     ISSN:  1573-4935     ISO Abbreviation:  Biosci. Rep.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2009-10-08     Completed Date:  2010-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102797     Medline TA:  Biosci Rep     Country:  United States    
Other Details:
Languages:  eng     Pagination:  33-40     Citation Subset:  IM    
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, People's Republic of China.
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MeSH Terms
Benzofurans / metabolism*
Cells, Cultured
Cinnamates / metabolism*
Depsides / metabolism*
Gene Expression Regulation, Plant / physiology
Molecular Structure
Plant Proteins / genetics,  metabolism
Plant Roots / cytology,  metabolism*
Salvia miltiorrhiza / cytology,  metabolism*
Silver / metabolism,  pharmacology
Stress, Physiological / drug effects,  physiology
Reg. No./Substance:
0/Benzofurans; 0/Cinnamates; 0/Depsides; 0/Plant Proteins; 121521-90-2/lithospermic acid B; 537-15-5/rosmarinic acid; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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