Document Detail

Lithium's Gene Expression Profile, Relevance to Neuroprotection A cDNA Microarray Study.
MedLine Citation:
PMID:  23324999     Owner:  NLM     Status:  Publisher    
Lithium can prevent 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) dopaminergic neurotoxicity in mice. This is attributed to induced antioxidant and antiapoptotic state, which among other factors results from induction of Bcl-2 and reduction of Bax, however, cDNA microarray reveals that this represents only one cascade of lithium targets. From analyzing the gene expression profile of lithium, we are able to point out candidate genes that might be involved in the antioxidant and neuroprotective properties of lithium. Among these are, the cAMP response element binding (CREB) protein, extracellular signal-regulated kinase (ERK), both CREB and ERK-part of the mitogen-activated kinase pathway-were upregulated by lithium, downregulated by MPTP, and maintained in mice fed with lithium chloride (LiCl) supplemented diet and treated with MPTP. Our positive control included tyrosine hydroxylase which both its mRNA and protein levels were independently measured, in addition to Bcl-2 protein levels. Other important genes which were similarly regulated are plasma glutathione peroxidase precursor (GSHPX-P), protein kinase C alpha type, insulin-like growth factor binding protein 4 precursor, and interferon regulatory factor. In addition, some genes were oppositely regulated, i.e., downregulated by lithium, upregulated by MPTP, and maintained in mice fed with LiCl supplemented diet and treated with MPTP, among these genes were basic fibroblast growth factor receptor 1 precursor, inhibin alpha subunit, glutamate receptor subunit zeta 1 precursor (NMD-R1), postsynaptic density protein-95 which together with NMD-R1 can form an apoptotic promoting complex. The discussed targets represent part of genes altered by chronic lithium. In fact lithium affected the expressions of more than 50 genes among these were basic transcription factors, transcription activators, cell signaling proteins, cell adhesion proteins, oncogenes and tumor suppressors, intracellular transducers, survival and death genes, and cyclins, here we discuss the relevance of these changes to lithium's reported neuroprotective properties.
Raymond Farah; Rola Khamisy-Farah; Tamar Amit; Moussa B H Youdim; Zaher Arraf
Related Documents :
11882459 - Discovery of osteoblast-associated genes using cdna microarrays.
7615639 - Identification and characterization of a fibroblast marker: fsp1.
20006559 - Effect of circumferential constraint on nucleus pulposus tissue in vitro.
18245529 - Prognostic value of ccn3 in osteosarcoma.
10529789 - New perspectives on the molecular basis of hereditary bone tumours.
18076769 - Unravelling the molecular control of calvarial suture fusion in children with craniosyn...
1524989 - Studies of the rb1 gene and the p53 gene in human osteosarcomas.
10563639 - A nonpathogenic gaagga repeat in the friedreich gene: implications for pathogenesis.
23294979 - The value of molecular expression of kit and kit ligand analysed using real-time polyme...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  -     ISSN:  1573-6830     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Internal Medicine B, Ziv Medical Center, Safed, Israel,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Retinal Cell Death Induced by TRPV1 Activation Involves NMDA Signaling and Upregulation of Nitric Ox...
Next Document:  A novel platform for enhanced biosensing based on the synergy effects of electrospun polymer nanofib...